Neutral Protease AF GMP Grade - Animal Free
For Cell Isolation from Pancreas, Adipose Tissue and Tumors
Neutral Protease AF GMP Grade is derived from C.histolyticum using a production process completely free of any animal-based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF GMP Grade in combination with highly purified Collagenase AF-1 GMP Grade. Here, it has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells.
|Molecular weight||34 kDa|
|Special features||Manufactured free from animal components & according to international GMP guidelines|
Sterility (Ph. Eur.)
Absence of Clostridia
TAMC (Total Aerobic Microbial Count) ≤ 10/Vial
TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial
|Activities||Neutral Protease activity: ≥ 0.50 U/mg lyophilisate (DMC assay**)|
|Storage conditions||+2 °C to +8 °C|
|Shipping conditions||At ambient temperature worldwide|
|Intended use||For clinical applications|
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Neutral Protease AF GMP Grade is often used in combination with Collagenase AF-1 GMP Grade. If your application is in the field of research, Nordmark provides the research grade alternatives Neutral Protease NB and Collagenase NB 1 Premium Grade.
|Product Name||Cat. No.||Size||Contact us||Order|
|Neutral Protease AF GMP Grade||N0003553||≥ 100 DMC*U||Inquire||Webshoplink|
Please contact us for the Certificate of Analysis!
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-642-4 • WGK: 1 • HS: 35079090 • EC 184.108.40.206 • CAS [9001-92-7]