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Neutral Protease AF GMP Grade - Animal Free

For Cell Isolation from Pancreas, Adipose Tissue and Tumors

Neutral Protease AF GMP Grade is derived from C.histolyticum using a production process completely free of any animal-based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF GMP Grade in combination with highly purified Collagenase AF-1 GMP Grade. Here, it has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells.

Product Information

OriginClostridium histolyticum
CompositionNeutral Protease
Molecular weight34 kDa 
Special featuresManufactured free from animal components & according to international GMP guidelines
Microbiological examinationEndotoxin*:
Sterility (Ph. Eur.)
Absence of Clostridia
TAMC (Total Aerobic Microbial Count) ≤ 10/Vial
TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial
ActivitiesNeutral Protease activity:  ≥ 0.50 U/mg lyophilisate (DMC assay**)
Storage conditions+2 °C to +8 °C
Shipping conditionsAt ambient temperature worldwide
Intended useFor clinical applications

Unit definition: 
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

**Neutral Protease: 
1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.

References:

  1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Related Products

Neutral Protease AF GMP Grade is often used in combination with Collagenase AF-1 GMP Grade. If your application is in the field of research, Nordmark provides the research grade alternatives Neutral Protease NB and Collagenase NB 1 Premium Grade

Ordering Information

Product NameCat. No. SizePrice
Neutral Protease AF GMP GradeN0003553≥ 100 DMC*UInquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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