Confocal microscopy of fibroblast cells

Isolation of Fibroblasts with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of fibroblasts from rodent and human skin and connective tissue are presented.


SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 4G Proved Grade 0.2 - 0.5 PZ U/ml, 
12 - 20 h, 37 °C
HumanResearchCollagenase NB 4G Proved Grade  0.26 PZ U/ml,
2 h, 37 °C
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml,
2 h, 37 °C

Help us Expand our Knowledge!

If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now! 

Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 4G Proved Grade
This product has an activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!