Several yellow adipocytes

Isolation of Adipocytes, ADSCs and SVF with Collagenase NB

Protocol Recommendations

 
In the following, application details for isolation of adipocytes from human or murine adipose tissue are presented.

Adipocytes & Adipose-Derived Stem Cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MurineResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml*
30 - 60 min, 37 °C
Details
HumanResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml*,
30 - 60 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml*,
30 - 60 min , 37 °C
Details

Stromal Vascular Fraction (Stem Cells) 

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanClinicCollagenase AF-1 GMP Grade &
Neutral Protease AF GMP Grade
0.4 PZ U/ml & 0.2 DMC U/ml**,
50 min, 37 °C
Details

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase AF-1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenase activity of  ≥ 3.00 U/mg.  It is produced under GMP guidelines and completely free of any animal-based components.

Neutral Protease AF GMP Grade
This neutral protease is a highly purified enzyme with a proteolytic activity of ≥ 0.50 DMC U/mg. It is produced under GMP guidelines and completely free of any animal-based components.

Collagenase NB 4 Standard Grade  
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us