Clostripain NB - The Third Component
For Pancreatic Islet Isolation and Other Dissociation
Clostripain NB is a highly purified enzyme originated from Clostridium histolyticum. The enzyme clostripain, also known as Clostridiopeptidase B or Endoproteinase Arg-C, is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation.
Clostripain exists in two different forms, an oxidized (native condition) and a reduced (activated form) that differ in enzymatic activity. Native clostripain can be activated with reducing agents (e.g. DTT, Cysteine), resulting in a higher activity of the enzyme.
Clostripain NB is used in combination with purified Collagenase NB 1 Premium Grade and/or Neutral Protease NB as well as Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range. For research use only.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains chromatographically highly purified clostripain |
| Molecular weight | 59 kDa |
| Special features | - |
| Microbiological examination | - |
| Activities | Clostripain, native state activity: ≥ 200 U/Vial (BAEE)* Clostripain, native state activity: ≥ 5.0 U/mg (BAEE)* Clostripain, activated activity: ≥ 50.0 U/mg (BAEE)** |
| Storage conditions | -15 °C to -25 °C |
| Shipping conditions | Delivered on dry ice |
| Intended use | For research use only |
Unit definition:
*Clostripain, native state (BAEE assay)
1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8.
**Clostripain, activated (BAEE assay)
1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8. The enzyme is activated with 2.5 mM DTT.
Related Products
Clostripain NB is often used in combination with Collagenase NB 1 Premium Grade and Neutral Protease NB. Clostripain NB can be used with Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range as well.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Clostripain NB | S2020501 | ≥ 200 U** | Inquire | Webshoplink |
Documentation
SDS Clostripain NB106 KB
CoO Clostripain415 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
