Micoskpic Image of isolated Islets

Clostripain NB - The Third Component

For Pancreatic Islet Isolation and Other Dissociation

Clostripain NB is a highly purified enzyme originated from Clostridium histolyticum. The enzyme clostripain, also known as Clostridiopeptidase B or Endoproteinase Arg-C, is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation.

Clostripain exists in two different forms, an oxidized (native condition) and a reduced (activated form) that differ in enzymatic activity. Native clostripain can be activated with reducing agents (e.g. DTT, Cysteine), resulting in a higher activity of the enzyme.

Clostripain NB is used in combination with purified Collagenase NB 1 Premium Grade and/or Neutral Protease NB as well as Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range. For research use only.

Product Information

OriginClostridium histolyticum
CompositionContains chromatographically highly purified clostripain
Molecular weight59 kDa
Special features-
Microbiological examination-
ActivitiesClostripain, native state activity: ≥ 200 U/Vial (BAEE)*
Clostripain, native state activity: ≥ 5.0 U/mg (BAEE)* 
Clostripain, activated activity: ≥ 50.0 U/mg (BAEE)** 
Storage conditions-15 °C to -25 °C
Shipping conditionsCooled on blue or dry ice depending on the destination
Intended useFor research use only

Unit definition: 

*Clostripain, native state (BAEE assay)
1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8.

**Clostripain, activated (BAEE assay)
1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8. The enzyme is activated with 2.5 mM DTT.

Related Products

Clostripain NB is often used in combination with Collagenase NB 1 Premium Grade and Neutral Protease NB. Clostripain NB can be used with Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range as well.

Ordering Information

Product NameCat. No. SizePrice
Clostripain NBS2020501≥ 200 U**Inquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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