Clostripain NB - The Third Component
For Pancreatic Islet Isolation and Other Dissociation
Clostripain NB is a highly purified enzyme originated from Clostridium histolyticum. The enzyme clostripain, also known as Clostridiopeptidase B or Endoproteinase Arg-C, is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation.
Clostripain exists in two different forms, an oxidized (native condition) and a reduced (activated form) that differ in enzymatic activity. Native clostripain can be activated with reducing agents (e.g. DTT, Cysteine), resulting in a higher activity of the enzyme.
Clostripain NB is used in combination with purified Collagenase NB 1 Premium Grade and/or Neutral Protease NB as well as Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range. For research use only.
|Composition||Contains chromatographically highly purified clostripain|
|Molecular weight||59 kDa|
|Activities||Clostripain, native state activity: ≥ 200 U/Vial (BAEE)*|
Clostripain, native state activity: ≥ 5.0 U/mg (BAEE)*
Clostripain, activated activity: ≥ 50.0 U/mg (BAEE)**
|Storage conditions||-15 °C to -25 °C|
|Shipping conditions||Cooled on blue or dry ice depending on the destination|
|Intended use||For research use only|
*Clostripain, native state (BAEE assay)
1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8.
**Clostripain, activated (BAEE assay)
1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8. The enzyme is activated with 2.5 mM DTT.
Clostripain NB is often used in combination with Collagenase NB 1 Premium Grade and Neutral Protease NB. Clostripain NB can be used with Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range as well.
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 18.104.22.168 • CAS [9001-12-1]