Different Organism Applications
Protocol Recommendations
In the following, application details for cell isolation from different organisms are presented.
Cartilage
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Bovine | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml, 14 - 16 h, 37 °C | Details |
Reproductive
Oocytes from Xenopus Laevis
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Frog | Research | Collagenase NB 4G Proved Grade | 0.35 - 0.5 PZ U/ml, 1.5 - 2 h, RT | Details |
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Brief Product Information
Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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