A young brown calf, cow, looking at the camera, with clean white sky, isolated

Different Organism Applications

Protocol Recommendations

In the following, application details for cell isolation from different organisms are presented.


SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
BovineResearchCollagenase NB 4 Standard Grade 0.2 - 0.3 PZ U/ml, 
14 - 16 h, 37 °C


Oocytes from Xenopus Laevis 

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
FrogResearchCollagenase NB 4G Proved Grade0.35 - 0.5 PZ U/ml,
1.5 - 2 h, RT

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Brief Product Information

Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 4 Standard Grade  
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us