3D illustration of a human heart

Isolation of Heart Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of cardiomyocytes from rodent and human heart tissue and endothelia cells from rodent aortas are presented.

Cardiomyocytes from cardiac muscle tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Rat (adult)ResearchCollagenase NB 8 Broad Range 0.5 PZ U/ml, 
45 - 60 min, 37 °C
Details
HumanResearch+Collagenase NB 8 Broad Range0.8 - 1.0 PZ U/ml, 
2 - 3.5 h, 37 °C
Details

Endothelia cells from aorta tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 4 Standard Grade 0.12 - 0.15 PZ U/ml, 
10 - 20 min, 37 °C
Details

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Brief Product Information

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!