Microscopic image of an acinic cell carcinoma

Isolation of Acinar Cells with Collagenase NB

Protocol Recommendations

 In the following, application details for isolation of acinar cells from human pancreatic tissue are presented.

Acinar Cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range0.05 - 0.1 PZ U/ml, 
1 h, 37 °C

+ For information on GMP grade Collagenase NB please contact us!

Help us Expand our Knowledge!

If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now! 

Brief Product Information

Collagenase NB 8 Broad Range  
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion, please contact us!