Isolation of Cardiomyocytes with Collagenase NB
In the following, application details for isolation of cardiomyocytes from rodent and human heart tissue are presented.
|Species||Application Field||Enzymes||Experimental Conditions||Downloads|
|Rat (adult)||Research||Collagenase NB 8 Broad Range||0.5 PZ U/ml, |
45 - 60 min, 37 °C
|Human||Research+||Collagenase NB 8 Broad Range||0.8 - 1.0 PZ U/ml, |
2 - 3.5 h, 37 °C
+For information on GMP grade Collagenase NB please contact us!
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Brief Product Information
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!