Isolation of Cardiomyocytes with Collagenase NB

Protocol Recommendations

 In the following, application details for isolation of cardiomyocytes from rodent and human heart tissue are presented.

Cardiomyocytes

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Rat (adult)ResearchCollagenase NB 8 Broad Range 0.5 PZ U/ml, 
45-60 min, 37 °C
Details
HumanResearch+Collagenase NB 8 Broad Range 0.8 - 1.0 PZ U/ml, 
2-3.5 h, 37 °C
Details

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Brief Product Information

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!