Isolation of Lung Cells with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of cells from murine lung and human lung tumor cells are presented.
Lung tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 4 Standard Grade | 0.2 PZ U/ml, 1 h, 37 °C | Details |
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Contact us |
+For information on GMP grade Collagenase NB please contact us!
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Brief Product Information
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!