3d rendered medically accurate illustration of the lung

Isolation of Lung Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of cells from murine lung and human lung tumor cells are presented.

Lung tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 4 Standard Grade0.5 PZ U/ml, 
45 - 60 min, 37 °C
Details
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
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Brief Product Information

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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