Collagenase NB 4 Standard Grade-For Research
For Dissociation of Cartilage, Umbilical Cord and More
Collagenase NB 4 Standard Grade is a natural enzyme mixture that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. As Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 5 Sterile Grade and Collagenase NB 6 GMP Grade, this enzyme may also be used in order to establish applications which have to meet sterile or GMP requirements like tissue engineering and transplantation.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains class I and class II collagenase 1 and a balanced ratio of proteolytic side activities |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | - |
| Microbiological examination | - |
| Activities | Collagenase activity: ≥ 0.10 U/mg (PZ activity* acc. to Wünsch, 25 °C) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For research use only |
*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Related Products
Collagenase NB 4 Standard Grade is often used to establish applications and to optimize protocols.If the application subsequently needs to meet sterile or GMP requirements, Collagenase NB 5 Sterile Grade or Collagenase NB 6 GMP Grade can be used. Both products have comparable enzymatic properties to Collagenase NB 4 Standard Grade
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase NB 4 Standard Grade | S1745402 | 500 mg | Inquire | Webshoplink |
| Collagenase NB 4 Standard Grade | S1745401 | 1 g | Inquire | Webshoplink |
| Collagenase NB 4 Standard Grade | S1745403 | 5 g | Inquire | Webshoplink |
Documentation
Product Info NB 453 KB
SDS NB 4108 KB
CoO NB 4 Standard Grade894 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
