3D model of stem cells

Collagenase NB 4 Standard Grade-For Research

For Dissociation of Cartilage, Umbilical Cord and More

Collagenase NB 4 Standard Grade is a natural enzyme mixture that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. As Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 5 Sterile Grade and Collagenase NB 6 GMP Grade, this enzyme may also be used in order to establish applications which have to meet sterile or GMP requirements like tissue engineering and transplantation.

Product Information

OriginClostridium histolyticum
CompositionContains class I and class II collagenase 1 and a balanced ratio of proteolytic side activities
Molecular weight70 – 120 kDa (collagenases)
Special features-
Microbiological examination-
ActivitiesCollagenase activity: ≥ 0.10 U/mg (PZ activity* acc. to Wünsch, 25 °C)
Storage conditions+2 °C to +8 °C
Shipping conditionsAt ambient temperature worldwide
Intended useFor research use only

*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:

  1.  Bond, M.D. & van Wart, H.E. (1984) Biochemistry 233077-3091
  2.  Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Related Products

Collagenase NB 4 Standard Grade is often used to establish applications and to optimize protocols.If the application subsequently needs to meet sterile or GMP requirements, Collagenase NB 5 Sterile Grade or Collagenase NB 6 GMP Grade can be used. Both products have comparable enzymatic properties to Collagenase NB 4 Standard Grade

 

Ordering Information

Product NameCat. No. SizePrice
Collagenase NB 4 Standard GradeS1745402500 mgInquire
Collagenase NB 4 Standard GradeS17454011 gInquire
Collagenase NB 4 Standard GradeS17454035 gInquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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