Isolation of Islets of Langerhans with Collagenase

Protocol Recommendations

 

In the following, application details for isolation of islets of Langerhans from human, rodent and porcine pancreatic tissue are presented.

Islets of Langerhans

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 1 Premium Grade &
Neutral Protease NB
Per gram tissue:
20 PZ U (NB 1),
0.8 - 1.5 DMC U (NP),
15 - 30 min, 37 °C
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HumanClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade
Per gram tissue:
20 PZ U (NB 1),
0.8 - 1.5 DMC U (NP),
15 - 30 min, 37 °C
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Porcine
(adult)
ResearchCollagenase NB 8 Broad Range &
Neutral Protease NB
Per gram tissue:
4.4 PZ U (NB 8),
0.5 - 0.7 DMC U (NP),
37 °C
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Porcine
(adult)
ClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade
Per gram tissue:
15 - 18  PZ U (NB 1),
0.8 - 1.3 DMC U (NP),
15 - 30 min, 37 °C
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Porcine
(neonatal)
ResearchCollagenase NB 4 Standard Grade0.17 - 0.2 PZ U/ml,
10 - 20 min, 37 °C
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Porcine
(neonatal)
ClinicCollagenase NB 6 GMP Grade0.17 - 0.2 PZ U/ml,
10 - 20 min, 37 °C
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RodentResearchCollagenase NB 8 Broad Range1.5 - 1.7 PZ U/ml
14 - 17 min, 37 °C
Details

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 1 Premium Grade
This product is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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