Protocol Recommendations
In the following, application details for isolation of islets of Langerhans from human, rodent and porcine pancreatic tissue are presented.
Islets of Langerhans
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 1 Premium Grade & Neutral Protease NB | Per gram tissue: 20 PZ U (NB 1), 0.8 - 1.5 DMC U (NP), 15 - 30 min, 37 °C | Contact us |
| Human | Clinic | Collagenase NB 1 GMP Grade & Neutral Protease NB GMP Grade | Per gram tissue: 20 PZ U (NB 1), 0.8 - 1.5 DMC U (NP), 15 - 30 min, 37 °C | Contact us |
| Porcine (adult) | Research | Collagenase NB 8 Broad Range & Neutral Protease NB | Per gram tissue: 4.4 PZ U (NB 8), 0.5 - 0.7 DMC U (NP), 37 °C | Details |
| Porcine (adult) | Clinic | Collagenase NB 1 GMP Grade & Neutral Protease NB GMP Grade | Per gram tissue: 15 - 18 PZ U (NB 1), 0.8 - 1.3 DMC U (NP), 15 - 30 min, 37 °C | Contact us |
| Porcine (neonatal) | Research | Collagenase NB 4 Standard Grade | 0.17 - 0.2 PZ U/ml, 10 - 20 min, 37 °C | Details |
| Porcine (neonatal) | Clinic | Collagenase NB 6 GMP Grade | 0.17 - 0.2 PZ U/ml, 10 - 20 min, 37 °C | Details |
| Rodent | Research | Collagenase NB 8 Broad Range | 1.5 - 1.7 PZ U/ml 14 - 17 min, 37 °C | Details |
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 1 Premium Grade
This product is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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