Collagenase NB 8 Broad Range-The Extra Push
For Cell Isolation of Rodent Pancreas and Tumors
Collagenase NB 8 Broad Range is a chromatographically purified enzyme resulting in a high collagenolytic activity. This product has an increased collagenase activity compared to Collagenase NB 4 Standard Grade, Collagenase NB 4G Proved Grade and Collagenase NB 7D Gentle Grade. The additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as the endotoxin level are reduced. Collagenase NB 8 is suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes.
|Composition||Contains class I and class II collagenase1 as well as reduced levels of clostripain, trypsin-like activities and neutral protease|
|Molecular weight||70 – 120 kDa (collagenases)|
|Microbiological examination||Endotoxin*: ≤ 100 EU/mg|
|Activities||Collagenase activity: ≥ 0.90 U/mg (PZ activity** acc. to Wünsch, 25 °C)|
|Storage conditions||+2 °C to +8 °C|
|Shipping conditions||At ambient temperature worldwide|
|Intended use||For research use only|
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 18.104.22.168 • CAS [9001-12-1]