3D model of group of isolated cancer cells

Collagenase NB 8 Broad Range-The Extra Push

For Cell Isolation of Rodent Pancreas and Tumors

Collagenase NB 8 Broad Range is a chromatographically purified enzyme resulting in a high collagenolytic activity. This product has an increased collagenase activity compared to Collagenase NB 4 Standard GradeCollagenase NB 4G Proved Grade and Collagenase NB 7D Gentle Grade. The additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as the endotoxin level are reduced. Collagenase NB 8 is suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes.

Product Information

OriginClostridium histolyticum
CompositionContains class I and class II collagenase1 as well as reduced levels of clostripain, trypsin-like activities and neutral protease
Chromatographically purified
Molecular weight70 – 120 kDa (collagenases)
Special features-
Microbiological examinationEndotoxin*: ≤ 100 EU/mg 
ActivitiesCollagenase activity: ≥ 0.90 U/mg (PZ activity** acc. to Wünsch, 25 °C)
Storage conditions+2 °C to +8 °C
Shipping conditions    At ambient temperature worldwide
Intended useFor research use only

Unit definition: 

*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

**PZ Unit (acc. to Wünsch2): 
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:

  1.  Bond, M.D. & van Wart, H.E. (1984) Biochemistry 233077-3091
  2.  Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Related Products

If you are dealing with poor cell yields, try collagenases with higher side activities like Collagenase NB 4 Standard Grade and Collagenase NB 4G Proved Grade.

Ordering Information

Product NameCat. No. SizePrice
Collagenase NB 8 Broad RangeS1745601250 mgInquire
Collagenase NB 8 Broad RangeS17456021 gInquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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