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Collagenase AF-1 GMP Grade- Animal Free

For Cell Isolation from Pancreas, Adipose Tissue and Tumors

Collagenase AF-1 GMP Grade is derived from C.histolyticum using a production process completely free of any animal-based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often used in combination with Neutral Protease AF GMP Grade, is suitable for the dissociation of sensitive cells from several tissues or organs such as pancreas, liver or tumors. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation, stromal vascular fraction (SVF) from adipose tissue and tumor cells.

 

Product Information

OriginClostridium histolyticum
CompositionCollagenase class I and class II1
Molecular weight70 – 120 kDa (collagenases)
Special featuresManufactured free from animal components & according to international GMP guidelines
Microbiological examinationEndotoxin*:
Sterility (Ph. Eur.)
Absence of Clostridia
TAMC (Total Aerobic Microbial Count) ≤ 10/Vial
TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial
ActivitiesCollagenase ≥ 150 U/Vial or ≥ 2000 U/Vial (PZ activity**according to Wünsch, 25 °C)
Collagenase ≥ 3000 U/mg lyophilisate (PZ activity** according to Wünsch, 25 °C)
Neutral Protease ≤ 0.050 U/mg lyophilisate (DMC assay***)
Storage conditions+2 °C to +8 °C
Shipping conditionsAt ambient temperature worldwide
Intended useFor clinical applications

Unit definition: 

*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

**PZ Unit (acc. to Wünsch2): 
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

***Neutral Protease: 
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS.3

References:

  1.  Bond, M.D. & van Wart, H.E. (1984) Biochemistry 233077-3091
  2.  Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
  3. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Related Products

Collagenase AF-1 GMP Grade is often used in combination with Neutral Protease AF GMP Grade. For research purposes, Nordmark provides the research grade alternatives Collagenase NB 1 Premium Grade and Neutral Protease NB.

Ordering Information

Product NameCat. No. SizePrice
Collagenase AF-1 GMP GradeN0003554≥ 2000 PZ U*Inquire
Collagenase AF-1 GMP GradeN0003855 ≥ 150 PZ U*Inquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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