Collagenase AF-1 GMP Grade- Animal Free
For Cell Isolation from Pancreas, Adipose Tissue and Tumors
Collagenase AF-1 GMP Grade is derived from C.histolyticum using a production process completely free of any animal-based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often used in combination with Neutral Protease AF GMP Grade, is suitable for the dissociation of sensitive cells from several tissues or organs such as pancreas, liver or tumors. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation, stromal vascular fraction (SVF) from adipose tissue and tumor cells.
|Composition||Collagenase class I and class II1|
|Molecular weight||70 – 120 kDa (collagenases)|
|Special features||Manufactured free from animal components & according to international GMP guidelines|
|Microbiological examination||Endotoxin* ≤ 10 EU/mg|
Absence of Clostridia
TAMC (Total Aerobic Microbial Count) ≤ 10/Vial
TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial
|Activities||Collagenase ≥ 150 U/Vial or ≥ 2000 U/Vial (PZ activity**according to Wünsch, 25 °C)|
Collagenase ≥ 3.000 U/mg lyophilisate (PZ activity** according to Wünsch, 25 °C)
Neutral Protease ≤ 0.050 U/mg lyophilisate (DMC assay***)
|Storage conditions||+2 °C to +8 °C|
|Shipping conditions||At ambient temperature worldwide|
|Intended use||For clinical applications|
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS.3
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Collagenase AF-1 GMP Grade is often used in combination with Neutral Protease AF GMP Grade. For research purposes, Nordmark provides the research grade alternatives Collagenase NB 1 Premium Grade and Neutral Protease NB.
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 126.96.36.199 • CAS [9001-12-1]