Collagenase AF-1 GMP Grade- Animal Free
For Cell Isolation from Pancreas, Adipose Tissue and Tumors
Collagenase AF-1 GMP Grade is derived from C.histolyticum using a production process completely free of any animal-based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often used in combination with Neutral Protease AF GMP Grade, is suitable for the dissociation of sensitive cells from several tissues or organs such as pancreas, liver or tumors. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation, stromal vascular fraction (SVF) from adipose tissue and tumor cells.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Collagenase class I and class II1 |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | Manufactured free from animal components & according to international GMP guidelines |
| Microbiological examination | Endotoxin* ≤ 10 EU/mg Absence of Clostridia TAMC (Total Aerobic Microbial Count) ≤ 10/Vial TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial |
| Activities | Collagenase ≥ 150 U/Vial or ≥ 2000 U/Vial (PZ activity**according to Wünsch, 25 °C) Collagenase ≥ 3.000 U/mg lyophilisate (PZ activity** according to Wünsch, 25 °C) Neutral Protease ≤ 0.050 U/mg lyophilisate (DMC assay***) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For clinical applications |
Unit definition:
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
**PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
***Neutral Protease:
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS.3
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Related Products
Collagenase AF-1 GMP Grade is often used in combination with Neutral Protease AF GMP Grade. For research purposes, Nordmark provides the research grade alternatives Collagenase NB 1 Premium Grade and Neutral Protease NB.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase AF-1 GMP Grade | N0003554 | ≥ 2000 PZ U* | Inquire | Webshoplink |
| Collagenase AF-1 GMP Grade | N0003855 | ≥ 150 PZ U* | Inquire | Webshoplink |
Documentation
SDS AF-1 GMP107 KB
CoO AF-1 GMP Grade444 KB
For the Certificate of Analysis please contact us!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
