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Isolation of Hepatocytes from Liver Tissue with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of hepatocytes from rodent, porcine and human liver tissues are presented.

Hepatocytes from liver tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4G Proved Grade 0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
HumanClinicCollagenase NB 6 GMP Grade0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
MouseResearchCollagenase NB 4G Proved Grade 0.08 - 0.11 PZ U/ml, 
5 - 15min, 37 °C
PigResearchCollagenase NB 8 Broad Range0.2 - 0.3 PZ U/ml
Rat (adult)ResearchCollagenase NB 4G Proved Grade 0.12 - 0.15 PZ U/ml, 
10 - 25 min, 37 °C

Human Liver Stem Cells (HLSC)

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanClinicCollagenase NB 1Neutral Protease NB0.6 mg/ml NB 1, 0.73 mg/ml NP, 37 ⁰CPublication

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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