3d illustration of Abstract medical background with Diseased liver

Isolation of Hepatocytes from Liver Tissue with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of hepatocytes from rodent, porcine and human liver tissues are presented.

Hepatocytes from liver tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4G Proved Grade 0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details
MouseResearchCollagenase NB 4G Proved Grade 0.08 - 0.11 PZ U/ml, 
5 - 15min, 37 °C
Details
PigResearchCollagenase NB 8 Broad Range0.2 - 0.3 PZ U/mlContact us
Rat (adult)ResearchCollagenase NB 4G Proved Grade 0.12 - 0.15 PZ U/ml, 
10 - 25 min, 37 °C
Details

Help us Expand our Knowledge!

If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now! 

Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!