Isolation of Hepatocytes from Liver Tissue with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of hepatocytes from rodent, porcine and human liver tissues are presented.
Hepatocytes from liver tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4G Proved Grade | 0.25 - 0.35 PZ U/ml, 10 - 20 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.25 - 0.35 PZ U/ml, 10 - 20 min, 37 °C | Details |
| Mouse | Research | Collagenase NB 4G Proved Grade | 0.08 - 0.11 PZ U/ml, 5 - 15min, 37 °C | Details |
| Pig | Research | Collagenase NB 8 Broad Range | 0.2 - 0.3 PZ U/ml | Details |
| Rat (adult) | Research | Collagenase NB 4G Proved Grade | 0.12 - 0.15 PZ U/ml, 10 - 25 min, 37 °C | Details |
Human Liver Stem Cells (HLSC)
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 1, Neutral Protease NB | 0.6 mg/ml NB 1, 0.73 mg/ml NP, 37 ⁰C | Publication |
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Collagenase NB 1 Premium Grade
This product is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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