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Rodent Applications

In the following, application details for cell isolation from rodent tissue are presented.

Adipose/Fat

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MurineResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml*
30 - 60 min, 37 °C
Details

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
 

Bone

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 4 Standard Grade0.15 - 0.25 PZ U/ml, 
5 - 10 min, 37 °C*
Details

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue

Cartilage

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
 Mouse
(newborn)
ResearchCollagenase NB 4 Standard Grade & Neutral Protease NB Consecutive
digestion steps:
0.5 - 1 DMC U/ml (NP),
 15 - 20 min, 37 °C
0.3 PZ U/ml (NB 4), 
30 - 45 min, 37 °C
Details

Gastric/Intestine

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
 RodentResearchCollagenase NB 4G Proved Grade0.1 - 0.2 PZ U/ml, 
30 - 60 min, 37 °C
Details

Heart

Cardiomyocytes from cardiac muscle tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Rat (adult)ResearchCollagenase NB 8 Broad Range 0.5 PZ U/ml, 
45 - 60 min, 37 °C
Details

 Endothelia cells from aorta tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 4 Standard Grade 0.12 - 0.15 PZ U/ml, 
10 - 20 min, 37 °C
Details

Liver

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 4G Proved Grade 0.08 - 0.11 PZ U/ml, 
5 - 15min, 37 °C
Details
Rat (adult)ResearchCollagenase NB 4G Proved Grade 0.12 - 0.15 PZ U/ml, 
10 - 25 min, 37 °C
Details

Lung

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 4 Standard Grade0.5 PZ U/ml, 
45 - 60 min, 37 °C
Details

Muscle

Cardiomyocytes from cardiac muscle tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Rat (adult)ResearchCollagenase NB 8 Broad Range 0.5 PZ U/ml, 
45 - 60 min, 37 °C
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Neural

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 4 Standard Grade 0.15 - 0.25 PZ U/ml, 
30 - 60 h, 37 °C
Details

Schwann cells from neuronal tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml,
1 - 3 h, 37 °C
Details

Pancreas

Islet of Langerhans from pancreatic tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 8 Broad Range1.5 - 1.7 PZ U/ml
14 - 17 min, 37 °C
Details

Reproductive

Epithelia cells from murine mammary gland

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 4 Standard Grade0.34 PZ U/ml, 
15 - 25 min, 37 °C
Details

Skin

Endothelia cells from rodent aortas

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 4 Standard Grade0.12 - 0.15 PZ U/ml, 
10 - 20 min, 37 °C
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Melanoma cells from murine skin 

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 8 Broad Range0.12 PZ U/ml,
30 min, 37 °C
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Fibroblasts from murine skin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
RodentResearchCollagenase NB 4G Proved Grade0.2 - 0.5 PZ U/ml, 
12 - 20 h, 37 °C
Details

Leukocytes from murine skin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MurineResearchCollagenase NB 7D Gentle Grade0.18 - 0.22 PZ U/ml,
30 min, 37 °C
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Tumor

Tumor cells from murine lung

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MurineResearchCollagenase NB 7D Gentle Grade

0.5 - 0.6 PZ U/ml,
1.5 - 2 h, 37 °C,  
add 100 μg/mL DNase

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Brief Product Information

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Collagenase NB 7D Gentle Grade
This product is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.

Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 4 Standard Grade  
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us