Rodent Applications
In the following, application details for cell isolation from rodent tissue are presented.
Adipose/Fat
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Murine | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
Bone
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 5 - 10 min, 37 °C* | Details |
* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
Cartilage
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse (newborn) | Research | Collagenase NB 4 Standard Grade & Neutral Protease NB | Consecutive digestion steps: 0.5 - 1 DMC U/ml (NP), 15 - 20 min, 37 °C 0.3 PZ U/ml (NB 4), 30 - 45 min, 37 °C | Details & Publication |
Gastric/Intestine
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4G Proved Grade | 0.1 - 0.2 PZ U/ml, 30 - 60 min, 37 °C | Details |
Heart
Cardiomyocytes from cardiac muscle tissue:
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rat (adult) | Research | Collagenase NB 8 Broad Range | 0.5 PZ U/ml, 45 - 60 min, 37 °C | Details |
Endothelia cells from aorta tissue:
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.12 - 0.15 PZ U/ml, 10 - 20 min, 37 °C | Details |
Liver
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 4G Proved Grade | 0.08 - 0.11 PZ U/ml, 5 - 15min, 37 °C | Details |
| Rat (adult) | Research | Collagenase NB 4G Proved Grade | 0.12 - 0.15 PZ U/ml, 10 - 25 min, 37 °C | Details |
Lung
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 4 Standard Grade | 0.2 PZ U/ml, 1 h, 37 °C | Details |
Muscle
Cardiomyocytes from cardiac muscle tissue:
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rat (adult) | Research | Collagenase NB 8 Broad Range | 0.5 PZ U/ml, 45 - 60 min, 37 °C | Contact us |
Neural
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 h, 37 °C | Details |
Schwann cells from neuronal tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml, 1 - 3 h, 37 °C | Details |
Pancreas
Islet of Langerhans from pancreatic tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 8 Broad Range | 1.5 - 1.7 PZ U/ml 14 - 17 min, 37 °C | Details |
Reproductive
Epithelia cells from murine mammary gland
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 4 Standard Grade | 0.34 PZ U/ml, 15 - 25 min, 37 °C | Details |
Skin
Endothelia cells from rodent aortas
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.12 - 0.15 PZ U/ml, 10 - 20 min, 37 °C | Contact us |
Melanoma cells from murine skin
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 8 Broad Range | 0.12 PZ U/ml, 30 min, 37 °C | Contact us |
Fibroblasts from murine skin
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4G Proved Grade | 0.2 - 0.5 PZ U/ml, 12 - 20 h, 37 °C | Details |
Leukocytes from murine skin
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Murine | Research | Collagenase NB 7D Gentle Grade | 0.18 - 0.22 PZ U/ml, 30 min, 37 °C | Contact us |
Tumor
Tumor cells from murine lung
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Murine | Research | Collagenase NB 7D Gentle Grade | 0.5 - 0.6 PZ U/ml, | Contact us |
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Brief Product Information
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 7D Gentle Grade
This product is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!