Collagenase NB 7D Gentle Grade-Gentle to Cells

For Dendritic Cells and Other Sensitive Tumor Cells

Collagenase NB 7D Gentle Grade is suitable for isolation of a broad variety of cells. Moreover, it can be used especially where sensitive conditions are favored, such as during isolation of dendritic and tumor cells from human or animal tissues. To meet these requirements, Collagenase NB 7D Gentle Grade combines the benefits of slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.

Product Information

OriginClostridium histolyticum
CompositionContains class I and class II1 collagenases and reduced trypsin-like activities
Molecular weight70 – 120 kDa (collagenases)
Special features-
Microbiological examination-
ActivitiesCollagenase activity: ≥ 0.2 U/mg lyophilisate (PZ activity* acc. to Wünsch, 25 °C)
Storage conditions+2 °C to +8 °C
Shipping conditionsAt ambient temperature worldwide
Intended useFor research use only

*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:

  1.  Bond, M.D. & van Wart, H.E. (1984) Biochemistry 233077-3091
  2.  Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Related Products

If you are dealing with less efficient cell isolation results, a product with higher tryptic activities like Collagenase NB 4G Proved Grade might be more suitable. However, in case of poor cell viability, try a product with even less tryptic activities like Collagenase NB 1 Premium Grade.

Ordering Information

Product NameCat. No. SizePrice
Collagenase NB 7D Gentle GradeS1747401500 mgInquire
Collagenase NB 7D Gentle GradeS17474021 gInquire
Collagenase NB 7D Gentle GradeS17474035 gInquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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