Collagenase NB 7D Gentle Grade-Gentle to Cells
For Dendritic Cells and Other Sensitive Tumor Cells
Collagenase NB 7D Gentle Grade is suitable for isolation of a broad variety of cells. Moreover, it can be used especially where sensitive conditions are favored, such as during isolation of dendritic and tumor cells from human or animal tissues. To meet these requirements, Collagenase NB 7D Gentle Grade combines the benefits of slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains class I and class II1 collagenases and reduced trypsin-like activities |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | - |
| Microbiological examination | - |
| Activities | Collagenase activity: ≥ 0.2 U/mg lyophilisate (PZ activity* acc. to Wünsch, 25 °C) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For research use only |
*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Related Products
If you are dealing with less efficient cell isolation results, a product with higher tryptic activities like Collagenase NB 4G Proved Grade might be more suitable. However, in case of poor cell viability, try a product with even less tryptic activities like Collagenase NB 1 Premium Grade.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase NB 7D Gentle Grade | S1747401 | 500 mg | Inquire | Webshoplink |
| Collagenase NB 7D Gentle Grade | S1747402 | 1 g | Inquire | Webshoplink |
| Collagenase NB 7D Gentle Grade | S1747403 | 5 g | Inquire | Webshoplink |
Documentation
CoO NB 7D Gentle Grade556 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
