3d rendered medically accurate illustration of bacteria inside of the intestine

Isolation of Tumor Cells fom colon tissue with Collagenase

Protocol Recommendations

In the following, application details for isolation of cells from colon tumor tissue presented.

Colon

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+ Collagenase NB 4G Proved Grade 0.5 - 1 PZ U/ml,
RT
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Brief Product Information

Collagenase NB 4G Proved Grade 
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!