Concept of Education anatomy and physiology of Hyaline cartilage under the microscopic in laboratory

Isolation of Chondrocytes with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of chondrocytes from murine, bovine and human cartilage are presented.

Chondrocytes

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
 Mouse
(newborn)
ResearchCollagenase NB 4 Standard Grade & Neutral Protease NB Consecutive
digestion steps:
0.5 - 1 DMC U/ml(NP),
 15 - 20 min, 37 °C
0.3 PZ U/ml (NB 4), 
30 - 45 min, 37 °C
Details & Publication
BovineResearchCollagenase NB 4 Standard Grade 0.2 - 0.3 PZ U/ml, 
14 - 16 h, 37 °C
Details
HumanResearchCollagenase NB 4 Standard Grade 0.3-0.4 PZ U/ml, 
4-16 h, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade 0.3 - 0.4 PZ U/ml,
4 - 16 h, 37 °C
Details

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 4 Standard Grade 
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.

 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!