Isolation of Muscle Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of cells from human skeletal muscle are presented.

Muscle Cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range0.4 - 0.6 PZ U/ml,
  37 °C
Details

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Brief Product Information

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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