Micoskpic Image of isolated Islets

Neutral Protease NB High Active Grade-Extra Strength

For Pancreatic Islet Isolation and Other Dissociation

Neutral Protease NB High Active Grade is a chromatographically purified metalloprotease suitable for different tissue dissociation applications. This product contains an increased proteolytic activity, is largely free from collagenolytic activity (PZ U) and has a very low endotoxin level. For cell isolation, it is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade.

Product Information

OriginClostridium histolyticum
CompositionContains highly active, chromatographically purified neutral protease from Clostridium histolyticum
Molecular weight34 kDa
Special features-
Microbiological examination    Endotoxin*: ≤ 50 EU/mg 
ActivitiesNeutral Protease activity: ≥ 4.00 U/mg (DMC assay**)
Storage conditions+2 °C to +8 °C
Shipping conditionsCooled on blue or dry ice depending on the destination
Intended useFor research use only

Unit definition: 
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

**Neutral Protease: 
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.

References:

  1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Related Products

Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade. If the application requires decreased proteolytic activity, Neutral Protease NB may be used. For applications under GMP requirements, Nordmark offers Neutral Protease NB GMP Grade.

Ordering Information

Product NameCat. No. SizePrice
Neutral Protease NB High Active GradeS303020280 DMC U*Inquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

Need Assistance?