Neutral Protease NB High Active Grade-Extra Strength
For Pancreatic Islet Isolation and Other Dissociation
Neutral Protease NB High Active Grade is a chromatographically purified metalloprotease suitable for different tissue dissociation applications. This product contains an increased proteolytic activity, is largely free from collagenolytic activity (PZ U) and has a very low endotoxin level. For cell isolation, it is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains highly active, chromatographically purified neutral protease |
| Molecular weight | 34 kDa |
| Special features | - |
| Microbiological examination | Endotoxin*: ≤ 50 EU/mg |
| Activities | Neutral Protease activity: ≥ 4.00 U/mg (DMC assay**) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For research use only |
Unit definition:
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
**Neutral Protease:
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.
References:
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Related Products
Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade. If the application requires decreased proteolytic activity, Neutral Protease NB may be used. For applications under GMP requirements, Nordmark offers Neutral Protease NB GMP Grade.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Neutral Protease NB High Active Grade | S3030202 | 80 DMC U* | Inquire | Webshoplink |
Documentation
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-822-2 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9028-00-6]
