Neutral Protease NB High Active Grade-Extra Strength
For Pancreatic Islet Isolation and Other Dissociation
Neutral Protease NB High Active Grade is a chromatographically purified metalloprotease suitable for different tissue dissociation applications. This product contains an increased proteolytic activity, is largely free from collagenolytic activity (PZ U) and has a very low endotoxin level. For cell isolation, it is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade.
|Composition||Contains highly active, chromatographically purified neutral protease from Clostridium histolyticum|
|Molecular weight||34 kDa|
|Microbiological examination||Endotoxin*: ≤ 50 EU/mg|
|Activities||Neutral Protease activity: ≥ 4.00 U/mg (DMC assay**)|
|Storage conditions||+2 °C to +8 °C|
|Shipping conditions||Cooled on blue or dry ice depending on the destination|
|Intended use||For research use only|
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade. If the application requires decreased proteolytic activity, Neutral Protease NB may be used. For applications under GMP requirements, Nordmark offers Neutral Protease NB GMP Grade.
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 188.8.131.52 • CAS [9001-12-1]