Isolation of Stem Cells with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of stem cells from human or murine adipose tissue, Whartons's Jelly, embryonic and mesenchymal stem cells are presented.
Stem Cells from Adipose Tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
| Human | Clinic | Collagenase AF-1 GMP Grade & Neutrale Protease AF GMP Grade | 0.4 PZ U/ml** & 0.2 DMC U/ml**, 50 min, 37 °C | Details |
| Murine | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
Stem Cells from Wharton's Jelly
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C | Details |
Human Embryonic Stem Cells (hESC)
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 6 GMP Grade | 1.25 mg/ml, 4min , 37 °C | Publication |
Human Liver Stem Cells (HLSC)
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 1, Neutral Protease NB | 0.6 mg/ml NB 1, 0.73 mg/ml NP, 37 ⁰C | Publication |
Mesenchymal Stem Cells
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 4G Proved Grade | 1 mg/ml (w/w), 37 ⁰C, 30 min | Publication |
| Human | Clinic | Collagenase NB 6 GMP Grade | 1 IU/ml with 1 IU/ml hyaluronidase, 2.5 hours, 37 ⁰C | Publication |
Neural Crest-derived Stem Cells
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Reserach | Collagenase NB 4 Standard Grade | 1.17 PZ U/ml, 1.5 -2 h, 37 ⁰C | Publication |
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase AF-1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenase activity of ≥ 3.00 U/mg. It is produced under GMP guidelines and completely free of any animal-based components.
Neutral Protease AF GMP Grade
This neutral protease is a highly purified enzyme with a proteolytic activity of ≥ 0.50 DMC U/mg. It is produced under GMP guidelines and completely free of any animal-based components.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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