3D model of stem cells

Isolation of Stem Cells with Collagenase NB

Protocol Recommendations

 In the following, application details for isolation of stem cells from human or murine adipose tissue are presented.

Stem Cells from Adipose Tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml*
30 - 60 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml*,
30 - 60 min , 37 °C
Details
HumanClinicCollagenase AF-1 GMP Grade &
Neutrale Protease AF GMP Grade

0.4 PZ U/ml** & 0.2 DMC U/ml**,

50 min, 37 °C

Details
MurineResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml*
30 - 60 min, 37 °C
Details

Stem Cells from Wharton's Jelly

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.13 - 0.15 PZ U/ml,
1 - 6 h , 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.13 - 0.15 PZ U/ml,
1 - 6 h , 37 °C
Details​​​​​​​

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue

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Brief Product Information

Collagenase NB 6 GMP Grade  
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase AF-1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenase activity of  ≥ 3.00 U/mg. It is produced under GMP guidelines and completely free of any animal-based components.

Neutral Protease AF GMP Grade
This neutral protease is a highly purified enzyme with a proteolytic activity of ≥ 0.50 DMC U/mg. It is produced under GMP guidelines and completely free of any animal-based components.

Collagenase NB 4 Standard Grade  
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us