Microscopic image of complex endometrial hyperplasi

Isolation of Endometrial Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of cells from human endometrium are presented.

Endomtrial cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade 0.2 - 0.3 PZ U/ml, 
30 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml, 
30 min, 37 °C
Details

Help us Expand our Knowledge!

If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now! 

Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!