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Isolation of Schwann Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of Schwann cells from nerve tissue are presented.

Schwann Cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade
0.5 PZ U/ml, 2 DCM U/ml, 
18 h, 37 °C
Details
RodentResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml,
1 - 3 h, 37 °C
Details​​​​​​​​​​​​​​

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Brief Product Information

Collagenase NB 1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. Research quality 
Collagenase NB 1 Premium Grade is also available.

Neutral Protease NB GMP Grade 
This neutral protease is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available in research quality Neutral Protease NB.

Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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