Isolation of Schwann Cells with Collagenase NB
In the following, application details for isolation of Schwann cells from nerve tissue are presented.
|Collagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade
|0.5 PZ U/ml, 2 DCM U/ml,
18 h, 37 °C
|Collagenase NB 4 Standard Grade
|0.2 - 0.3 PZ U/ml,
1 - 3 h, 37 °C
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Brief Product Information
Collagenase NB 1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. Research grade
Collagenase NB 1 Premium Grade is also available.
Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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