Fluorescence Microscope image of Bovine Pulmonary Artery Endothelial Cells

Isolation of Endothelial Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of endothelial cells from rodent aortas, human foreskin (HDMEC) and umbilical cord (HUVEC) are presented.

Endothelial Cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
 RodentResearchCollagenase NB 4 Standard Grade 0.12 - 0.15 PZ U/ml, 
10 - 20 min, 37 °C
Details

Human
(HDMEC)

ResearchCollagenase NB 4 Standard Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
Human
(HDMEC)
ClinicCollagenase NB 6 GMP Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
 Human
 (HUVEC)
ResearchCollagenase NB 4 Standard Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
Human
(HUVEC)
ClinicCollagenase NB 6 GMP Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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