Neurons cells concept

Isolation of Neurons with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of neurons from human and rodent dorsal root ganglia and Schwann cells from nerve tissue are presented.

Neural

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °CDetails
HumanClinicCollagenase NB 6 GMP Grade 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °CDetails
RodentResearchCollagenase NB 4 Standard Grade 0.15 - 0.25 PZ U/ml, 
30-60 h, 37 °C
Details

 Schwann cells from neuronal tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade 
0.5 PZ U/ml, 2 DCM U/ml, 
18 h, 37 °C
Details
RodentResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml,
1-3 h, 37 °C
Details

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 1 GMP Grade 
This collagenase is a highly purified enzyme with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. Research quality Collagenase NB 1 Premium Grade is also available.

Neutral Protease NB GMP Grade 
This neutral protease is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available in research quality Neutral Protease NB.

Collagenase NB 4 Standard Grade 
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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