Muscle Type: Skeletal muscle - Cross section through a muscle with visible muscle fibers - 3D Rendering

Isolation of Muscle Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of cells from human skeletal muscle and murine or human cardiomyocytes are presented.

Cardiomyocytes from Cardiac Muscle Tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Rat (adult)ResearchCollagenase NB 8 Broad Range 0.5 PZ U/ml, 
45 - 60 min, 37 °C
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HumanResearch+Collagenase NB 8 Broad Range0.8 - 1.0 PZ U/ml, 
2 - 3.5 h, 37 °C
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Myocytes from Skeletal Suscle Tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range0.4 - 0.6 PZ U/ml,
37 °C
Details

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Brief Product Information

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!