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Publications

Publications with Collagenase NB

Publications on Acinar Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Cathepsin B Activity Initiates Apoptosis via Digestive Protease Activation in Pancreatic Acinar Cells and Experimental PancreatitisSendler, M. et al. J. Biol. Chem. 2016, 291, 14717-14731.Acinar cellsPancreasRodentn.d.*n.d.*

 *not defined

Publications on Adipocytes

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Signaling pathways involved in LPS induced TNFalpha production in human adipocytesHoareau, L. et al. Journal Inflamm.2010, 7.AdipocytesAdipose TissueHumanCollagenase NB 41.5 mg/ml; 0.175 PZ U/mg, 30 min, 37 ⁰C
Ceiling Culture-Derived Proliferative Adipocytes are A Possible Delivery Vehicle for Enzyme Replacement Therapy in Lecithin: Cholesterol Acyltransferase DeficiencyKuroda, M. et al. Open Gene Ther. J. 2011, 4, 1-10.AdipocytesAdipose TissueHumanCollagenase NB 62 mg/ml

 

Publications on Adipose Stem Cells (ASC)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Autologous fat transfer with in-situ mediation AIM a novel and compliant method of adult mesenchymal stem cell therapyWu, A. Y. and Morrow, D. J. Translat. Med. 2013, 11.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 60.1 PZ U/ml
Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cellsPatrikoski, M. et al. Stem Cell Res Ther. 2013, 4, 27.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 637 °C
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem CellsCarvalho,P. et al. Tiss Eng Part C Methods 2013, 19.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 40.2 PZ U/ml, 1 h, 37 ⁰C 
Human Adipose-Derived Mesenchymal Stem Cells in Cell Therapy: Safety and Feasibility in Different "Hospital Exemption" Clinical ApplicationsVeriter, S. et al. Plos One 2015, 10, e0139566.Adipose stem cells (ASC)Adipose TissueHumann.d.*> 8 PZ U/ml, 60 min, 37 ⁰C 
A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical ApplicationsRaposio, E. et al. Annals Plast. Surg. 2015, 00.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 62 mg/ml, 30 min, 37 ⁰C

*not defined

Publication on Bone Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Use of Bioengineered Bone for Sinus AugmentationBeaumont, C. et al. J Periodontol 2008, 79, 541-548.Bone PeriosteumHumanCollagenase NB 6n.d.*

*not defined

Publication on Brain Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
A non‑aggressive, highly efficient, enzymatic method for dissociation of human brain‑tumors and brain‑tissues to viable single‑cellsVolovitz, I. et al. BMC Neurosci. 2016, 17, 1-10.Brain CellsBrainHumanNeutral Protease0.11 DMC U/m

Publication of Cerebral Endothelial Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Cerebral endothelial cells release TNF-alpha after stimulation with cell walls of Streptococcus pneumoniae and regulate inducible nitric oxide synthase and ICAM-1 expression via autocrine loopsFreyer, D. et al. J. Immunol. 1999, 163, 4308-4314.Cerebral endothelial cellsBrainRodentsn.d.*1.1 PZ U/ml, 37 ⁰C,  1 h

*not defined

Publication of Chondrozytes

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Atoh8 acts as a regulator of chondrocyte proliferation and differentiation in endochondral bonesSchroeder, N. et al. Plos One 2019, 14, e0218230.ChondrozytesLimb budsRodentNeutral Protease, Collagenase NB 4Sequential digestion:
1) 1 DMC U/ml, 15 min, 37 ⁰C
2) 0.3 PZ U/ml, 30 min, 37 ⁰C

Publications on Hepatocytes

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Severe hepatic injury in interleukin 18 (IL-18) transgenic mice: a key role for IL-18 in regulating hepatocyte apoptosis in vivoFinotto,S. et al. Gut 2004, 53, 392-400.HepatocytesLiverRodentn.d.*n.d.*
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivoAurich, H. et al. Gut 2009, 58, 570-581.HepatocytesLiverRodentCollagenase NB 4G0.75 mg/ml
Cold Storage of Rat Hepatocyte Suspensions for One Week in a Customized Cold Storage Solution – Preservation of Cell Attachment and MetabolismPless-Petig, G. et al. PlosOne 2012, 7, e40444.HepatocytesLiverRodentCollagenase NB 450 PZ U/L
Comparison of methods for isolating primary hepatocytes from mini pigsLi, Y. et al. Xenotransplantation2016, 0, 1-7.HepatocytesLiverPorcineCollagenase NB 4, Collagenase NB 8

120 PZ U/L, 35 min, 37 ⁰C

*not defined

Publications on Human Embryonic Stem Cells (hESC)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Good manufacturing practice and clinical-grade human embryonic stem cell linesUnger, C. Human Mol. Gen. 2008, 17, R48-R53.Human embryonic stem cells (hESC)n.d.*Humann.d.*n.d.*
Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studiesHentze, H. et al. Stem Cell Res. 2009, 2, 198-210.Human embryonic stem cells (hESC)Blastocyst-stage preimplantation embryosHumanCollagenase NB 61.25 mg/ml,   4 min

*not defined

Publications on Human Liver Stem Cells (HLSC)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Intrahepatic Administration of Human Liver Stem Cells in Infants with Inherited Neonatal-Onset Hyperammonemia: A Phase I StudySpada, M. et al. Stem Cell Rev Rep. 2020, 16, 186-197.Human Liver Stem Cells (HLSC)LiverHumanCollagenase NB 1, Neutral Protease0.6 mg/ml NB 1, 0.73 mg/ml NP, 37 ⁰C

Publications on Human Umbilical Vein Endothelial Cells (HUVEC)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banksPolchow, B. et al. J Transl Med. 2012, 10, 98.Human umbilical vein endothelial cells (HUVEC)Umbilical cordHumanCollagenase NB 6

0.12 PZ U/ml, 20 min

Publications on Islets of Langerhans

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Successful Single Donor Islet Allotransplantation in the Streptozotocin Diabetes Rat ModelJahr, H. Cell Transplant. 2002, 11, 513-518.Islets of LangerhansPancreasRodentn.d.*1.7 mg/ml
Tyrosine hydroxylase activity in the endocrine pancreas: changes induced by short-term dietary manipulationBorelli, M. I. et al. BMC Endocr Disord. 2003, 3.Islets of LangerhansPancreasRodentn.d.*n.d.*
Influence of Collagenase Loading on Long-Term Preservation of Pig Pancreas by the Two-Layer Method for Subsequent Islet IsolationBrandhorst, D. et al. Transplant. 2005, 79, 38-43.Islets of LangerhansPancreasPorcineCollagenase NB 8, Neutral Protease NBn.d.*
Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.Brandhorst, D. et al. Xenotransplant. 2006, 13, 465-470.Islets of LangerhansPancreasPorcine Collagenase NB 8, Neutral Protease NBn.d.*
Current status of pancreatic islet transplantationMerani, S. and Shaprio, A. M. J. Clin Sci (Lond.) 2006, 110, 611-625.Islets of LangerhansPancreasHuman Collagenase NB 1, Neutral Proteasen.d.*
Quantification of Basal and Stimulated ROS Levels as Predictors of Islet Potency and FunctionArmann, B et al. Am J Transplant. 2007, 7, 38-47.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.d.*
Factors Influencing the Collagenase Digestion Phase of Human Islet IsolationKin, T. et al. Transplant.2007, 83, 7-12.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.d.*
Improvement of pancreatic islet cell isolation for transplantationMatsumoto,S. et al. Proc (Bayl Univ Med Cent.) 2007, 20, 357-562.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.d.*
Collagenase penetrates human pancreatic islets following standard intraductal administrationCross, S.E. Transplant. 2008, 86, 907-911.Islets of LangerhansPancreasHumanCollagenase NB 1,Neutral Proteasen.d.*
Iodixanol-Controlled Density Gradient During Islet Purification Improves Recovery Rate in Human Islet IsolationNoguchi, H. et al. Transplant. 2009, 87, 1629-1635.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NBn.d.*
Pig Pancreas Oxygenation at 20°C Increases Islet ATP Generation but Deteriorates Islet FunctionIken, m. et al. Cell Transplant. 2009, 18, 745-751.Islets of LangerhansPancreasPorcineCollagenase NB 8Neutral Protease NB4.4 PZ U/ml and 0.9-1.1 DMC U/ml, 30⁰C - 37⁰C
The Importance of Tryptic-like Activity in Purified Enzyme Blends for Efficient Islet IsolationBrandhorst, H. et al. Transplant. 2009, 87, 370-375.Islets of LangerhansPancreas Rodent and Human Collagenase NB 1, Neutral Protease NBn.d.*
Human Islet Isolation for Autologous Transplantation: Comparison of Yield and Function Using SERVA/Nordmark Versus Roche EnzymesAnazawa,T. et al. Am J Transplant. 2009, 9, 2383-2391.Islets of LangerhansPancreas Human Collagenase NB 1, Neutral Protease NBn.d.*
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic TissueQi, M. J Vis Exp. 2009, 26, 1125-1126.Islets of LangerhansPancreasHumann.d.*n.d.*
Low-Temperature Preservation of Isolated Islets is Superior to Conventional Islet Culture Before Islet TransplantationNoguchi, H. et al. Transplant. 2010, 89, 47-54.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NB20 PZ U/ml and 1 DMC U/ml,  37 ⁰C
Experience of islet isolation without neutral protease supplementationKin, T. et al. Islets, 2010, 2, 278-282.Islets of LangerhansPancreasHumanCollagenase NB 1 GMP, Neutral Protease NB GMPn.d.*
Islet Isolation for Clinical TransplantationKin, T. Adv Exp Med Biol. 2010, 654, 683-710.Islets of LangerhansPancreasHumann.d.*n.d.*.
A Novel Device for Islte Transplantation Providing Immune Protection and Oxygen SupplyLudwig, B. et al.Horm Metab Res. 2010, 42, 918-922.Islets of LangerhansPancreasHumanCollagenase NB 8n.d.*
Agonist of growth hormone-releasing hormone as a potential effector for survival and proliferation of pancreatic isletsLudwig, B. et al. PNAS 2010, 107, 12623-12628.Islets of LangerhansPancreasHumanCollagenase NB 1,Neutral Proteasen.d.*
Assessment of human islet isolation with four different collagenasesShimoda,M. et al. Transplant Proc. 2010, 42, 2049-2051.Islets of LangerhansPancreasHumanCollagenase NB 1n.d.*
Seven consecutive successful clinical islet isolations with pancreatic ductal injectionMatsumoto, S. et al. Cell Transplant. 2010, 19, 291-297.Islets of LangerhansPancreasHumanCollagenase NB 1,Neutral Proteasen.d.*
Large-scale Comparison of Libearse HI and Collagenase NB 1 utilized for Himan Islet IsolationBrandhorst,H. et al. Cell Transplant. 2010, 19, 3-8.Islets of LangerhansPancreas Human Collagenase NB 1, Neutral Protease NBn.d.*
A Simplified Approach to Human Islet Quality AssessmentHanson, M. S. et al. Transplant. 2010, 89, 1178-1188.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NBn.d.*
Evolution of β-Cell Replacement Therapy in DiabetesMellitus: Islet Cell TransplantationJahansouz, C. et al. Diabetes Technol Ther. 2011, 13, 395-418.Islets of LangerhansPancreasHumann.d.*n.d.*
Recent progress in pancreatic islet transplantationKuise, t. and Noguchi, H. World J Transplant. 2011, 1, 13-18.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NBn.d.*
Impact of the number of infusions on 2-year results of islet-after-kidney transplantation in the GRAGIL networkBorot,S et al. Transplant. 2011, 92, 1031-1038.Islets of LangerhansPancreas HumanCollagenase NB 1n.d.*
Improved Pancreatic Islet Isolation Outcome in Autologous Transplantation for Chronic PancreatitisNaziruddin, B. et al Cell Transplant 2012, 21, 553-558.Islets of LangerhansPancreas HumanCollagenase NB 1, Neutral Protease NBn.d.*
Comparative impact on islet isolation and transplant outcome of the preservation solutions Institut Georges Lopez-1, University of Wisconsin, and CelsiorNiclauss, N. et al. Transplant. 2012, 93, 703-708.Islets of LangerhansPancreas HumanCollagenase NB 1, Neutral Protease NBn.d.*
Fresh Islets Are More Effective for Islet Transplantation Than Cultured IsletsNoguchi, H. et al. Cell Transplant. 2012, 21, 517-523.Islets of LangerhansPancreas HumanCollagenase NB 1, Neutral Protease NB20 PZ U/ml and 1 DMC U/ml,  37 ⁰C
Evaluation of Osmolality of Density Gradient for Human Islet PurificationNoguchi, H. et al. Cell Transplant. 2012, 21, 493-500.Islets of LangerhansPancreas HumanCollagenase NB 1, Neutral Protease NB20 PZ U/ml and 1 DMC U/ml,  37 ⁰C
Impact of Tissue Volume and Purification on Clinical Autologous Islet Transplantation for the Treatment of Chronic PancreatitisMatsumoto,S. et al. Cell Transplant. 2012, 21, 625-632.Islets of LangerhansPancreasHumanCollagenase NB 1Neutral Protease NBn.d.*
Update on Islet TransplantationMcCall, M. and Shapiro, A. M. J. Cold Spring Harb Perspect Med. 2012, 2, a007823.Islets of LangerhansPancreasHumanCollagenase NB 1Neutral Protease NBn.d.*
Improvement of collagenase distribution with the ductal preservation for human islet isolationShimoda, M. et al. Islets 2012, 4, 130-137.Islets of LangerhansPancreasHumann.d.*n.d.*
Isolation of human islets for autologous islet transplantation in children and adolescents with chronic pancreatitisBottino,R et al. J Transplant. 2012, 642787.Islets of LangerhansPancreas Humann.d.*n.d.*
Collagenase Does Not Persist in Human Islets Following IsolationCross, S. E. Cell Transplant. 2012, 21, 2531-2535.Islets of LangerhansPancreas HumanCollagenase NB 1, Neutral Protease NBn.d.*
Transplanted Functional Islet Mass: Donor, Islet Preparation, and Recipient Factors Influence Early Graft Function in Islet-After-Kidney PatientsFriberg, A. S. et al. Transplant. 2012, 93, 632-638.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NBn.d.*
Robot-assisted pancreatoduodenectomy with preservation of the vascular supply for autologous islet cell isolation and transplantation: a case reportGiulianotti, P. et al J Med case rep. 2012, 6.Islets of LangerhansPancreasHumann.d.*n.d.*
Quality of Isolated Pig Islets Is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe ModelBrandhorst, H. et al. Cell Transplant. 2013, 22, 1477-1483.Islets of LangerhansPancreas Porcine Collagenase NB 1, Neutral Protease NBn.d.*
Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociationO'Gorman, D. et al. Islets, 2013, 5, 111-115.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NB High Activen.d.*
Transplantation of Encapsulated Pancreatic Islets as a Treatment for Patients with Type 1 Diabetes MellitusQi, M. Adv Med. 2014, 429710.Islets of LangerhansPancreasHumanCollagenase NB 1Neutral Protease1600 - 2285 PZ U and 200 - 286 DMC units, 14 min
Human islet cell isolation: the initial step in an islet transplanting program in Shiraz, Southern IranAzarpira,N. et al. Exp Clin Transplant.2014, 12, 139-142.Islets of LangerhansPancreas HumanCollagenase NB 1n.d.*
Effects of Donor-, Pancreas-, and Isolation-Related Variables on Human Islet Isolation Outcome: A Systematic ReviewHilling, D. et al. Cell Transplant. 2014, 23, 921-928.Islets of LangerhansPancreasHumanCollagenase NB 1n.d.*
Islet Product Characteristics and Factors Related to Successful Human Islet Transplantation From the Collaborative Islet Transplant Registry (CITR) 1999–2010Balamurugan, A.N. et al. Am J Transplant 2014, 14, 2595-2606.Islets of LangerhansPancreasHumanCollagenase NB 1n.d.*
Autologous Islet Transplantation With Remote Islet Isolation After Pancreas Resection for Chronic PancreatitisTai, D.S. et al. JAMA Surg. 2015, 150, 118-124.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.d.*
National Institutes of Health–Sponsored Clinical Islet Transplantation Consortium Phase 3 Trial: Manufacture of a Complex Cellular Product at Eight Processing FacilitiesRicordi, C. et al. Diabetes 2016, 65, 3418-3428.Islets of LangerhansPancreasHuman

Collagenase NB 1,
Neutral Protease

n.d.*
Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?Brandhorst, D. et al. Rev Diabet Stud, 2016, 14, 22-38.Islets of LangerhansPancreasHumann.d.*n.d.*
Quantifying the Effects of Different Neutral Proteases on Human Islet IntegrityBrandhorst, H. et al. Cell Transplant 2017, 26, 1733-1741.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease, Clostripainn.d.*
Evaluation of collagenase gold plus BP protease in isolating islets from human pancreataKhiatah, B. Islets 2018, 4, 51-59.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease2542 PZ U/ml; 266 PZ U/ml
Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet IsolationLoganathan, G. et al. OBM Transplantation 2019, 3.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.d.*
Hypoxia-Preconditioned Wharton’s Jelly-Derived Mesenchymal
Stem Cells Mitigate Stress-Induced Apoptosis and Ameliorate Human Islet Survival and Function in Direct Contact Coculture System
Keshtkar, S. et al. StemCell Int. 2020.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.d.*
Comparison of islet isolation result and clinical applicability according to GMP-grade collagenase enzyme blend in adult porcine islet isolation and cultureKwak, K. et al. Xenotransplant. 2021, e12703.Islets of LangerhansPancreasPorcineCollagenase AF-1, Neutral Protease AFn.d.*

*not defined

Publications on Mesenchymal Stem Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivoAurich, H. et al. Gut 2009, 58, 570-581.Mesenchymal Stem CellsAdipose TissueHumanCollagenase NB 4G1 mg/ml (w/w), 37 ⁰C, 30 min
Nonexpanded mesenchymal stem cells for regenerative medicine - yield in stromal vascular fraction from adipose-tissueFaustini, M. et al. Tissue Engin. 2010, 16, 1515-1521.Mesenchymal Stem CellsAdipose TissueHumann.d.*n.d.*
A new standardized clinical-grade protocol for banking human umbilical cord tissue cellsFazzina, R. et al. Transfusion 2015, 00, 1-10.Mesenchymal Stem CellsUmbilical CordHumanCollagenase NB 61 IU/ml, 
together with 1 IU/ml hyaluronidase, 2.5 hours, 37 ⁰C

*not defined

Publications on Neural Crest-derived Stem Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeuticsGreiner, J. et al. Eur. Cells Mat. 2011, 22, 403-419.Neural crest-derived stem cellsNasal inferior turbinatesHumanCollagenase NB 41.17 PZ U/ml, 1.5 h, 37 ⁰C
Culture bag systems for clinical applications of adult human neural crest-derived stem cellsGreiner, J. et al. Stem Cell Res Ther. 2014, 5, 34.Neural crest-derived stem cellsNasal inferior turbinatesHumanCollagenase NB 41.17 PZ U/ml, 2 h, 37 ⁰C

Publications on Schwann Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatmentJin,Y-Q. et al. J Neurosci Methods 2008, 170, 140-148.Schwann CellsSciatic nervesRodentCollagenase NB 40.27 PZ U/ml

Publications on Stem Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
The use of glandular-derived stem cells to improve vascularization in scaffold-mediated dermal regenerationEgana, J. T. et al. Biomterials 2009, 30, 5918-5926.Stem cellsPancreas, Submandibular GlandsMouseCollagenase NB 8n.d.*
Glandular Stem Cells: A New Source for Myocardial Repair?Guldner, N. W. et al. Chapter in Reg. Medicine and Tiss. Eng. - Cells and Biomat. 2011 Stem cellsPancreasHumann.d.*0.63 PZ U/ml
Cellular modulation of polymeric device surfaces - promise of adult stem cells for neuro-prostheticsRichter, A. et al. Front Neurosci. 2011, 5, 1-10.Stem cellsPancreasRodentn.d.*0.63 PZ U/ml

*not defined

Publications on Stromal Vascular Fraction

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Validation of an Automated Procedure to Isolate Human Adipose Tissue–Derived Cells by Using the Sepax  TechnologyGüven, S. et al. Tissue Engin. 2012, 18, 575-582.Stromal Vascular FractionAdipose TissueHumanCollagenase NB 60.12 PZ U/ml, 1 h, 37 ⁰C
Development and Validation of a Fully GMP-Compliant Process for Manufacturing Stromal Vascular Fraction - A Cost-Effective Alternative to Automated MethodsFrancois, P. Cells 2020, 9, 2158-2182.Stromal Vascular FractionAdipose TissueHumanCollagenase NB 60.25 PZ U/ml, 45 min, 37 ⁰C

Publication of Pancreatic Stellate-Like Cells (PSLCs)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Pluripotency of adult stem cells derived from human and rat pancreasKruse,C. et al. Appl Phys.2004Pancreatic stellate-like cells (PSLCs)PancreasHumann.d.*0.63 PZ U/mg, 37 ⁰C, 15 min

*not defined

Publications on Tumor Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Phenotypic heterogeneity among tumorigenic melanoma cells from patients that is reversible and not hierarchically organizedQuintana, E. et al. Cancer Cell 2010, 5, 510-523.Tumor cellsMelanomaHumanCollagenase NB 65 ug/ml, 3 h, 37 ⁰C
A non‑aggressive, highly efficient, enzymatic method for dissociation of human brain‑tumors and brain‑tissues to viable single‑cellsVolovitz, I. et al. BMC Neurosci. 2016, 17,1-10.Tumor cellsBrainHumanNeutral Protease NB 0.11 DMC U/ml

Nordmark Biochemicals' Publication List

TitleReference Cell TypeTissueSpeciesEnzymes
Comparison of Islet Characterization from Use of Standard Crude Collagenase to GMP-Grade Collagenase Enzyme Blends in Preweaned Porcine Islet IsolationsCorrales, N. CellTransplant. 2020, 29, 1-11.           Islets of LangerhansPancreasPorcineCollagenase AF-1, Collagenase NB 6Neutral Protease AF
Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet IsolationBrandhorst, H. Cell Transplant. 2019, 28, 176-184.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NB, Clostripain
Multicentre Assessment Of Animal-Free Collagenase Af-1 For Human Islet IsolationBrandhorst, D. Cell Transplant. 2017, 26, 1688-1693.Islets of LangerhansPancreasHumanCollagenase AF-1, Neutral Protease AF
Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet IsolationBrandhorst, H. Transplant Direct, 2016, 2, e47.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NB, Clostripain
The effect of truncated collagenase class I isomers on human islet isolation outcomeBrandhorst, H. Transplantation 2010, 90, 334-335.Islets of LangerhansPancreasHumanCollagenase NB 1
Degraded collagenase deteriorates islet viabilityBrandhorst, D. Transplant. Proc. 2008, 40, 370-371.Islets of LangerhansPancreasRodent Collagenase NB 1
The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreasBrandhorst, H. Transplantation 2008, 85, 456-461.Islets of LangerhansPancreasRodent Collagenase NB 1
Adjustment of the ratio between collagenase class II and I improves islet isolation outcomeBrandhorst, D. Transplant. Proc. 2005, 37, 3450-3451.Islets of LangerhansPancreasRodent Collagenase NB 1
Assessment of a novel two-component enzyme preparation for human islet isolation and transplantationBucher, P. Transplanation, 2005, 79, 91-97.Islets of LangerhansPancreasHumanCollagenase NB 1
The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreasBrandhorst, H. Transplant Prod. 2005, 37, 215-216.Islets of LangerhansPancreasRodent Collagenase NB 1
Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolationBucher, P. Transplant Proc.2004, 36, 1145-1146.Islets of LangerhansPancreasRodent Collagenase NB 1
Serva collagenase NB1: a new enzyme preparation for human islet isolationBucher, P. Transplant Proc. 2004, 36, 1143-1144.Islets of LangerhansPancreasHumanCollagenase NB 1