Publications about Collagenase NB - Nordmark Pharma GmbH

Publications with Collagenase NB

Publications on Acinar Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Cathepsin B Activity Initiates Apoptosis via Digestive Protease Activation in Pancreatic Acinar Cells and Experimental Pancreatitis	Sendler, M. et al. J. Biol. Chem. 2016, 291, 14717-14731.	Acinar cells	Pancreas	Rodent	n.d.*	n.d.*

*not defined

Publications on Adipocytes

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Signaling pathways involved in LPS induced TNFalpha production in human adipocytes	Hoareau, L. et al. Journal Inflamm.2010, 7.	Adipocytes	Adipose Tissue	Human	Collagenase NB 4	1.5 mg/ml; 0.175 PZ U/mg, 30 min, 37 ⁰C
Ceiling Culture-Derived Proliferative Adipocytes are A Possible Delivery Vehicle for Enzyme Replacement Therapy in Lecithin: Cholesterol Acyltransferase Deficiency	Kuroda, M. et al. Open Gene Ther. J. 2011, 4, 1-10.	Adipocytes	Adipose Tissue	Human	Collagenase NB 6	2 mg/ml

Publications on Adipose Stem Cells (ASC)

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Autologous fat transfer with in-situ mediation AIM a novel and compliant method of adult mesenchymal stem cell therapy	Wu, A. Y. and Morrow, D. J. Translat. Med. 2013, 11.	Adipose stem cells (ASC)	Adipose Tissue	Human	Collagenase NB 6	0.1 PZ U/ml
Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells	Patrikoski, M. et al. Stem Cell Res Ther. 2013, 4, 27.	Adipose stem cells (ASC)	Adipose Tissue	Human	Collagenase NB 6	37 °C
GMP-level adipose stem cells combined with computer-aided manufacturing to reconstruct mandibular ameloblastoma resection defects: Experience with three cases	Wolff, J. et al. Ann Maxillofac Surg.2013, 3, 114-125.	Adipose stem cells (ASC)	Adipose Tissue	Human	Collagenase NB 6	n.d.*
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells	Carvalho,P. et al. Tiss Eng Part C Methods 2013, 19.	Adipose stem cells (ASC)	Adipose Tissue	Human	Collagenase NB 4	0.2 PZ U/ml, 1 h, 37 ⁰C
Human Adipose-Derived Mesenchymal Stem Cells in Cell Therapy: Safety and Feasibility in Different "Hospital Exemption" Clinical Applications	Veriter, S. et al. Plos One 2015, 10, e0139566.	Adipose stem cells (ASC)	Adipose Tissue	Human	n.d.*	> 8 PZ U/ml, 60 min, 37 ⁰C
A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical Applications	Raposio, E. et al. Annals Plast. Surg. 2015, 00.	Adipose stem cells (ASC)	Adipose Tissue	Human	Collagenase NB 6	2 mg/ml, 30 min, 37 ⁰C

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Publication on Bone Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Use of Bioengineered Bone for Sinus Augmentation	Beaumont, C. et al. J Periodontol 2008, 79, 541-548.	Bone	Periosteum	Human	Collagenase NB 6	n.d.*

*not defined

Publication on Brain Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
A non‑aggressive, highly efficient, enzymatic method for dissociation of human brain‑tumors and brain‑tissues to viable single‑cells	Volovitz, I. et al. BMC Neurosci. 2016, 17, 1-10.	Brain Cells	Brain	Human	Neutral Protease	0.11 DMC U/m

Publication of Cerebral Endothelial Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Cerebral endothelial cells release TNF-alpha after stimulation with cell walls of Streptococcus pneumoniae and regulate inducible nitric oxide synthase and ICAM-1 expression via autocrine loops	Freyer, D. et al. J. Immunol. 1999, 163, 4308-4314.	Cerebral endothelial cells	Brain	Rodents	n.d.*	1.1 PZ U/ml, 37 ⁰C, 1 h

*not defined

Publication of Chondrozytes

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Atoh8 acts as a regulator of chondrocyte proliferation and differentiation in endochondral bones	Schroeder, N. et al. Plos One 2019, 14, e0218230.	Chondrozytes	Limb buds	Rodent	Neutral Protease, Collagenase NB 4	Sequential digestion: 1) 1 DMC U/ml, 15 min, 37 ⁰C 2) 0.3 PZ U/ml, 30 min, 37 ⁰C

Publications on Hepatocytes

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Severe hepatic injury in interleukin 18 (IL-18) transgenic mice: a key role for IL-18 in regulating hepatocyte apoptosis in vivo	Finotto,S. et al. Gut 2004, 53, 392-400.	Hepatocytes	Liver	Rodent	n.d.*	n.d.*
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo	Aurich, H. et al. Gut 2009, 58, 570-581.	Hepatocytes	Liver	Rodent	Collagenase NB 4G	0.75 mg/ml
Cold Storage of Rat Hepatocyte Suspensions for One Week in a Customized Cold Storage Solution – Preservation of Cell Attachment and Metabolism	Pless-Petig, G. et al. PlosOne 2012, 7, e40444.	Hepatocytes	Liver	Rodent	Collagenase NB 4	50 PZ U/L
Comparison of methods for isolating primary hepatocytes from mini pigs	Li, Y. et al. Xenotransplantation2016, 0, 1-7.	Hepatocytes	Liver	Porcine	Collagenase NB 4, Collagenase NB 8	120 PZ U/L, 35 min, 37 ⁰C
Primary Murine Hepatocyte Isolation and Culture with GMP and Research-Grade Enzyme Blends	Whaley, D. et al. Jpn. J Gastroenterol Hepatol. 2022, V8(20), 1-7	Hepatocytes	Liver	Rodent	Collagenase NB 4G, Collagenase NB 6	0,125 PZ U/ml

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Publications on Human Embryonic Stem Cells (hESC)

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Good manufacturing practice and clinical-grade human embryonic stem cell lines	Unger, C. Human Mol. Gen. 2008, 17, R48-R53.	Human embryonic stem cells (hESC)	n.d.*	Human	n.d.*	n.d.*
Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studies	Hentze, H. et al. Stem Cell Res. 2009, 2, 198-210.	Human embryonic stem cells (hESC)	Blastocyst-stage preimplantation embryos	Human	Collagenase NB 6	1.25 mg/ml, 4 min

*not defined

Publications on Human Liver Stem Cells (HLSC)

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Intrahepatic Administration of Human Liver Stem Cells in Infants with Inherited Neonatal-Onset Hyperammonemia: A Phase I Study	Spada, M. et al. Stem Cell Rev Rep. 2020, 16, 186-197.	Human Liver Stem Cells (HLSC)	Liver	Human	Collagenase NB 1, Neutral Protease	0.6 mg/ml NB 1, 0.73 mg/ml NP, 37 ⁰C

Publications on Human Umbilical Vein Endothelial Cells (HUVEC)

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks	Polchow, B. et al. J Transl Med. 2012, 10, 98.	Human umbilical vein endothelial cells (HUVEC)	Umbilical cord	Human	Collagenase NB 6	0.12 PZ U/ml, 20 min

Publications on Islets of Langerhans

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Successful Single Donor Islet Allotransplantation in the Streptozotocin Diabetes Rat Model	Jahr, H. Cell Transplant. 2002, 11, 513-518.	Islets of Langerhans	Pancreas	Rodent	n.d.*	1.7 mg/ml
Tyrosine hydroxylase activity in the endocrine pancreas: changes induced by short-term dietary manipulation	Borelli, M. I. et al. BMC Endocr Disord. 2003, 3.	Islets of Langerhans	Pancreas	Rodent	n.d.*	n.d.*
Influence of Collagenase Loading on Long-Term Preservation of Pig Pancreas by the Two-Layer Method for Subsequent Islet Isolation	Brandhorst, D. et al. Transplant. 2005, 79, 38-43.	Islets of Langerhans	Pancreas	Porcine	Collagenase NB 8, Neutral Protease NB	n.d.*
Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.	Brandhorst, D. et al. Xenotransplant. 2006, 13, 465-470.	Islets of Langerhans	Pancreas	Porcine	Collagenase NB 8, Neutral Protease NB	n.d.*
Current status of pancreatic islet transplantation	Merani, S. and Shaprio, A. M. J. Clin Sci (Lond.) 2006, 110, 611-625.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Quantification of Basal and Stimulated ROS Levels as Predictors of Islet Potency and Function	Armann, B et al. Am J Transplant. 2007, 7, 38-47.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Factors Influencing the Collagenase Digestion Phase of Human Islet Isolation	Kin, T. et al. Transplant.2007, 83, 7-12.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Improvement of pancreatic islet cell isolation for transplantation	Matsumoto,S. et al. Proc (Bayl Univ Med Cent.) 2007, 20, 357-562.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Collagenase penetrates human pancreatic islets following standard intraductal administration	Cross, S.E. Transplant. 2008, 86, 907-911.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1,Neutral Protease	n.d.*
Robotic-Assisted Laparoscopic Distal Pancreatectomy of a Redo Case Combined With Autologous Islet Transplantation for Chronic Pancreatitis	Guiulianotti, P. C. et al.Pancreas2009, 38, 105-107.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Iodixanol-Controlled Density Gradient During Islet Purification Improves Recovery Rate in Human Islet Isolation	Noguchi, H. et al. Transplant. 2009, 87, 1629-1635.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Pig Pancreas Oxygenation at 20°C Increases Islet ATP Generation but Deteriorates Islet Function	Iken, m. et al. Cell Transplant. 2009, 18, 745-751.	Islets of Langerhans	Pancreas	Porcine	Collagenase NB 8, Neutral Protease NB	4.4 PZ U/ml and 0.9-1.1 DMC U/ml, 30⁰C - 37⁰C
The Importance of Tryptic-like Activity in Purified Enzyme Blends for Efficient Islet Isolation	Brandhorst, H. et al. Transplant. 2009, 87, 370-375.	Islets of Langerhans	Pancreas	Rodent and Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Human Islet Isolation for Autologous Transplantation: Comparison of Yield and Function Using SERVA/Nordmark Versus Roche Enzymes	Anazawa,T. et al. Am J Transplant. 2009, 9, 2383-2391.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue	Qi, M. J Vis Exp. 2009, 26, 1125-1126.	Islets of Langerhans	Pancreas	Human	n.d.*	n.d.*
Low-Temperature Preservation of Isolated Islets is Superior to Conventional Islet Culture Before Islet Transplantation	Noguchi, H. et al. Transplant. 2010, 89, 47-54.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	20 PZ U/ml and 1 DMC U/ml, 37 ⁰C
Experience of islet isolation without neutral protease supplementation	Kin, T. et al. Islets, 2010, 2, 278-282.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Islet Isolation for Clinical Transplantation	Kin, T. Adv Exp Med Biol. 2010, 654, 683-710.	Islets of Langerhans	Pancreas	Human	n.d.*	n.d.*.
A Novel Device for Islet Transplantation Providing Immune Protection and Oxygen Supply	Ludwig, B. et al.Horm Metab Res. 2010, 42, 918-922.	Islets of Langerhans	Pancreas	Human	Collagenase NB 8	n.d.*
Agonist of growth hormone-releasing hormone as a potential effector for survival and proliferation of pancreatic islets	Ludwig, B. et al. PNAS 2010, 107, 12623-12628.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1,Neutral Protease	n.d.*
Assessment of human islet isolation with four different collagenases	Shimoda,M. et al. Transplant Proc. 2010, 42, 2049-2051.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1	n.d.*
Seven consecutive successful clinical islet isolations with pancreatic ductal injection	Matsumoto, S. et al. Cell Transplant. 2010, 19, 291-297.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1,Neutral Protease	n.d.*
Large-scale Comparison of Libearse HI and Collagenase NB 1 utilized for Himan Islet Isolation	Brandhorst,H. et al. Cell Transplant. 2010, 19, 3-8.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
A Simplified Approach to Human Islet Quality Assessment	Hanson, M. S. et al. Transplant. 2010, 89, 1178-1188.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Evolution of β-Cell Replacement Therapy in DiabetesMellitus: Islet Cell Transplantation	Jahansouz, C. et al. Diabetes Technol Ther. 2011, 13, 395-418.	Islets of Langerhans	Pancreas	Human	n.d.*	n.d.*
Highly Purified versus Filtered Crude Collagenase: Comparable Human Islet Isolation Outcomes	Wang, Y. et al. Cell Transplant. 2011, 20, 817-1825.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1	n.d.*
Recent progress in pancreatic islet transplantation	Kuise, t. and Noguchi, H. World J Transplant. 2011, 1, 13-18.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Impact of the number of infusions on 2-year results of islet-after-kidney transplantation in the GRAGIL network	Borot,S et al. Transplant. 2011, 92, 1031-1038.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1	n.d.*
Improved Pancreatic Islet Isolation Outcome in Autologous Transplantation for Chronic Pancreatitis	Naziruddin, B. et al Cell Transplant 2012, 21, 553-558.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Comparative impact on islet isolation and transplant outcome of the preservation solutions Institut Georges Lopez-1, University of Wisconsin, and Celsior	Niclauss, N. et al. Transplant. 2012, 93, 703-708.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Fresh Islets Are More Effective for Islet Transplantation Than Cultured Islets	Noguchi, H. et al. Cell Transplant. 2012, 21, 517-523.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	20 PZ U/ml and 1 DMC U/ml, 37 ⁰C
Evaluation of Osmolality of Density Gradient for Human Islet Purification	Noguchi, H. et al. Cell Transplant. 2012, 21, 493-500.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	20 PZ U/ml and 1 DMC U/ml, 37 ⁰C
Impact of Tissue Volume and Purification on Clinical Autologous Islet Transplantation for the Treatment of Chronic Pancreatitis	Matsumoto,S. et al. Cell Transplant. 2012, 21, 625-632.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Update on Islet Transplantation	McCall, M. and Shapiro, A. M. J. Cold Spring Harb Perspect Med. 2012, 2, a007823.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Improvement of collagenase distribution with the ductal preservation for human islet isolation	Shimoda, M. et al. Islets 2012, 4, 130-137.	Islets of Langerhans	Pancreas	Human	n.d.*	n.d.*
Isolation of human islets for autologous islet transplantation in children and adolescents with chronic pancreatitis	Bottino,R et al. J Transplant. 2012, 642787.	Islets of Langerhans	Pancreas	Human	n.d.*	n.d.*
Collagenase Does Not Persist in Human Islets Following Isolation	Cross, S. E. Cell Transplant. 2012, 21, 2531-2535.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Transplanted Functional Islet Mass: Donor, Islet Preparation, and Recipient Factors Influence Early Graft Function in Islet-After-Kidney Patients	Friberg, A. S. et al. Transplant. 2012, 93, 632-638.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB	n.d.*
Robot-assisted pancreatoduodenectomy with preservation of the vascular supply for autologous islet cell isolation and transplantation: a case report	Giulianotti, P. et al J Med case rep. 2012, 6.	Islets of Langerhans	Pancreas	Human	n.d.*	n.d.*
Quality of Isolated Pig Islets Is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model	Brandhorst, H. et al. Cell Transplant. 2013, 22, 1477-1483.	Islets of Langerhans	Pancreas	Porcine	Collagenase NB 1, Neutral Protease NB	n.d.*
Clinical Islet Isolation Outcomes with a Highly Purified Neutral Protease for Pancreas Dissociation	O'Gorman, D. et al. Islets, 2013, 5, 111-115.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB High Active	n.d.*
Transplantation of Encapsulated Pancreatic Islets as a Treatment for Patients with Type 1 Diabetes Mellitus	Qi, M. Adv Med. 2014, 429710.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	1600 - 2285 PZ U and 200 - 286 DMC units, 14 min
Human islet cell isolation: the initial step in an islet transplanting program in Shiraz, Southern Iran	Azarpira,N. et al. Exp Clin Transplant.2014, 12, 139-142.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1	n.d.*
Effects of Donor-, Pancreas-, and Isolation-Related Variables on Human Islet Isolation Outcome: A Systematic Review	Hilling, D. et al. Cell Transplant. 2014, 23, 921-928.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1	n.d.*
Islet Product Characteristics and Factors Related to Successful Human Islet Transplantation From the Collaborative Islet Transplant Registry (CITR) 1999–2010	Balamurugan, A.N. et al. Am J Transplant 2014, 14, 2595-2606.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1	n.d.*
Autologous Islet Transplantation With Remote Islet Isolation After Pancreas Resection for Chronic Pancreatitis	Tai, D.S. et al. JAMA Surg. 2015, 150, 118-124.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
National Institutes of Health–Sponsored Clinical Islet Transplantation Consortium Phase 3 Trial: Manufacture of a Complex Cellular Product at Eight Processing Facilities	Ricordi, C. et al. Diabetes 2016, 65, 3418-3428.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?	Brandhorst, D. et al. Rev Diabet Stud, 2016, 14, 22-38.	Islets of Langerhans	Pancreas	Human	n.d.*	n.d.*
Effect of Manufacturing Procedures on Human Islet Isolation From Donor Pancreata Standardized by the North American Islet Donor Score	Yeh, C.-C. et al. Cell Transplant. 2017, 26, 33-44.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Quantifying the Effects of Different Neutral Proteases on Human Islet Integrity	Brandhorst, H. et al. Cell Transplant 2017, 26, 1733-1741.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease, Clostripain	n.d.*
Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata	Khiatah, B. Islets 2018, 4, 51-59.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	2542 PZ U/ml; 266 PZ U/ml
Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation	Loganathan, G. et al. OBM Transplantation 2019, 3.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Hypoxia-Preconditioned Wharton’s Jelly-Derived Mesenchymal Stem Cells Mitigate Stress-Induced Apoptosis and Ameliorate Human Islet Survival and Function in Direct Contact Coculture System	Keshtkar, S. et al. StemCell Int. 2020.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease	n.d.*
Comparison of islet isolation result and clinical applicability according to GMP-grade collagenase enzyme blend in adult porcine islet isolation and culture	Kwak, K. et al. Xenotransplant. 2021, e12703.	Islets of Langerhans	Pancreas	Porcine	Collagenase AF-1, Neutral Protease AF	n.d.*

*not defined

Publications on Mesenchymal Stem Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo	Aurich, H. et al. Gut 2009, 58, 570-581.	Mesenchymal Stem Cells	Adipose Tissue	Human	Collagenase NB 4G	1 mg/ml (w/w), 37 ⁰C, 30 min
Nonexpanded mesenchymal stem cells for regenerative medicine - yield in stromal vascular fraction from adipose-tissue	Faustini, M. et al. Tissue Engin. 2010, 16, 1515-1521.	Mesenchymal Stem Cells	Adipose Tissue	Human	n.d.*	n.d.*
A new standardized clinical-grade protocol for banking human umbilical cord tissue cells	Fazzina, R. et al. Transfusion 2015, 00, 1-10.	Mesenchymal Stem Cells	Umbilical Cord	Human	Collagenase NB 6	1 IU/ml, together with 1 IU/ml hyaluronidase, 2.5 hours, 37 ⁰C

*not defined

Publications on Neural Crest-derived Stem Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeutics	Greiner, J. et al. Eur. Cells Mat. 2011, 22, 403-419.	Neural crest-derived stem cells	Nasal inferior turbinates	Human	Collagenase NB 4	1.17 PZ U/ml, 1.5 h, 37 ⁰C
Culture bag systems for clinical applications of adult human neural crest-derived stem cells	Greiner, J. et al. Stem Cell Res Ther. 2014, 5, 34.	Neural crest-derived stem cells	Nasal inferior turbinates	Human	Collagenase NB 4	1.17 PZ U/ml, 2 h, 37 ⁰C

Publications on Schwann Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment	Jin,Y-Q. et al. J Neurosci Methods 2008, 170, 140-148.	Schwann Cells	Sciatic nerves	Rodent	Collagenase NB 4	0.27 PZ U/ml

Publications on Stem Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
The use of glandular-derived stem cells to improve vascularization in scaffold-mediated dermal regeneration	Egana, J. T. et al. Biomterials 2009, 30, 5918-5926.	Stem cells	Pancreas, Submandibular Glands	Mouse	Collagenase NB 8	n.d.*
Glandular Stem Cells: A New Source for Myocardial Repair?	Guldner, N. W. et al. Chapter in Reg. Medicine and Tiss. Eng. - Cells and Biomat. 2011	Stem cells	Pancreas	Human	n.d.*	0.63 PZ U/ml
Cellular modulation of polymeric device surfaces - promise of adult stem cells for neuro-prosthetics	Richter, A. et al. Front Neurosci. 2011, 5, 1-10.	Stem cells	Pancreas	Rodent	n.d.*	0.63 PZ U/ml

*not defined

Publications on Stromal Vascular Fraction

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Validation of an Automated Procedure to Isolate Human Adipose Tissue–Derived Cells by Using the Sepax Technology	Güven, S. et al. Tissue Engin. 2012, 18, 575-582.	Stromal Vascular Fraction	Adipose Tissue	Human	Collagenase NB 6	0.12 PZ U/ml, 1 h, 37 ⁰C
Development and Validation of a Fully GMP-Compliant Process for Manufacturing Stromal Vascular Fraction - A Cost-Effective Alternative to Automated Methods	Francois, P. Cells 2020, 9, 2158-2182.	Stromal Vascular Fraction	Adipose Tissue	Human	Collagenase NB 6	0.25 PZ U/ml, 45 min, 37 ⁰C

Publication of Pancreatic Stellate-Like Cells (PSLCs)

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Pluripotency of adult stem cells derived from human and rat pancreas	Kruse,C. et al. Appl Phys. A 2004, 79, 1617-1624.	Pancreatic stellate-like cells (PSLCs)	Pancreas	Human	n.d.*	0.63 PZ U/mg, 37 ⁰C, 15 min

*not defined

Publications on Tumor Cells

Title	Reference	Cell Type	Tissue	Species	Collagenase Product	Experimental Conditions
Phenotypic heterogeneity among tumorigenic melanoma cells from patients that is reversible and not hierarchically organized	Quintana, E. et al. Cancer Cell 2010,5, 510-523.	Tumor cells	Melanoma	Human	Collagenase NB 6	5 ug/ml, 3 h, 37 ⁰C
A non‑aggressive, highly efficient, enzymatic method for dissociation of human brain‑tumors and brain‑tissues to viable single‑cells	Volovitz, I. et al. BMC Neurosci. 2016,17,1-10.	Tumor cells	Brain	Human	Neutral Protease NB	0.11 DMC U/ml
Tumor-associated hematopoietic stem and progenitor cells positively linked to glioblastoma progression	Lu, I.-N. et al. Nat. Commun. 2021, 12, 3895.	Tumor cells	Brain	Human	Neutral Protease NB	n.d.*

*not defined

Nordmark Biochemicals' Publication List

Title	Reference	Cell Type	Tissue	Species	Enzymes
Comparison of Islet Characterization from Use of Standard Crude Collagenase to GMP-Grade Collagenase Enzyme Blends in Preweaned Porcine Islet Isolations	Corrales, N. CellTransplant. 2020, 29, 1-11.	Islets of Langerhans	Pancreas	Porcine	Collagenase AF-1, Collagenase NB 6, Neutral Protease AF
Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation	Brandhorst, H. Cell Transplant. 2019, 28, 176-184.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB, Clostripain
Multicentre Assessment Of Animal-Free Collagenase Af-1 For Human Islet Isolation	Brandhorst, D. Cell Transplant. 2017, 26, 1688-1693.	Islets of Langerhans	Pancreas	Human	Collagenase AF-1, Neutral Protease AF
Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation	Brandhorst, H. Transplant Direct, 2016, 2, e47.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1, Neutral Protease NB, Clostripain
The effect of truncated collagenase class I isomers on human islet isolation outcome	Brandhorst, H. Transplantation 2010, 90, 334-335.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1
Degraded collagenase deteriorates islet viability	Brandhorst, D. Transplant. Proc. 2008, 40, 370-371.	Islets of Langerhans	Pancreas	Rodent	Collagenase NB 1
The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas	Brandhorst, H. Transplantation 2008, 85, 456-461.	Islets of Langerhans	Pancreas	Rodent	Collagenase NB 1
Adjustment of the ratio between collagenase class II and I improves islet isolation outcome	Brandhorst, D. Transplant. Proc. 2005, 37, 3450-3451.	Islets of Langerhans	Pancreas	Rodent	Collagenase NB 1
Assessment of a novel two-component enzyme preparation for human islet isolation and transplantation	Bucher, P. Transplanation, 2005, 79, 91-97.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1
The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas	Brandhorst, H. Transplant Prod. 2005, 37, 215-216.	Islets of Langerhans	Pancreas	Rodent	Collagenase NB 1
Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation	Bucher, P. Transplant Proc.2004, 36, 1145-1146.	Islets of Langerhans	Pancreas	Rodent	Collagenase NB 1
Serva collagenase NB1: a new enzyme preparation for human islet isolation	Bucher, P. Transplant Proc. 2004, 36, 1143-1144.	Islets of Langerhans	Pancreas	Human	Collagenase NB 1