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Publications

Publications with Collagenase NB

Publications on Acinar Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Cathepsin B Activity Initiates Apoptosis via Digestive Protease Activation in Pancreatic Acinar Cells and Experimental PancreatitisSendler, M. et al. J. Biol. Chem. 2016, 291, 14717-14731.Acinar cellsPancreasRodentn.a.n.a.

 

Publications on Adipocytes

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Signaling pathways involved in LPS induced TNFalpha production in human adipocytesHoareau, L. et al. Journal Inflamm.2010, 7.AdipocytesAdipose TissueHumanCollagenase NB 41.5 mg/ml; 0.175 PZ U/mg, 30 min, 37 ⁰C
Ceiling Culture-Derived Proliferative Adipocytes are A Possible Delivery Vehicle for Enzyme Replacement Therapy in Lecithin: Cholesterol Acyltransferase DeficiencyKuroda, M. et al. Open Gene Ther. J. 2011, 4, 1-10.AdipocytesAdipose TissueHumanCollagenase NB 62 mg/ml

 

Publications on Adipose Stem Cells (ASC)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Autologous fat transfer with in-situ mediation AIM a novel and compliant method of adult mesenchymal stem cell therapyWu, A. Y. and Morrow, D. J. Translat. Med. 2013, 11.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 60.1 PZ U/ml
Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cellsPatrikoski, M. et al. Stem Cell Res Ther. 2013, 4, 27.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 637 °C
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem CellsCarvalho,P. et al. Tiss Eng Part C Methods2013, 19.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 40.2 PZ U/ml, 1 h, 37 ⁰C 
A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical ApplicationsRaposio, E. et al. Annals Plast. Surg. 2015, 00.Adipose stem cells (ASC)Adipose TissueHumanCollagenase NB 62 mg/ml, 30 min, 37 ⁰C

 

Publication on Bone Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Use of Bioengineered Bone for Sinus AugmentationBeaumont, C. et al. J Periodontol 2008, 79, 541-548.Bone PeriosteumHumanCollagenase NB 6n.a.

Publication on Brain Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
A non‑aggressive, highly efficient, enzymatic method for dissociation of human brain‑tumors and brain‑tissues to viable single‑cellsVolovitz, I. et al. BMC Neurosci. 2016, 17, 1-10.Brain CellsBrainHumanNeutral Protease0.11 DMC U/m

Publication of Chondrozytes

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Atoh8 acts as a regulator of chondrocyte proliferation and differentiation in endochondral bonesSchroeder, N. et al. Plos One 2019, 14, e0218230.ChondrozytesLimb budsRodentNeutral Protease, Collagenase NB 4Sequential digestion:
1) 1 DMC U/ml, 15 min, 37 ⁰C
2) 0.3 PZ U/ml, 30 min, 37 ⁰C

Publications on Hepatocytes

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivoAurich, H. et al. Gut 2009, 58, 570-581.HepatocytesLiverRodentCollagenase NB 4G0.75 mg/ml
Cold Storage of Rat Hepatocyte Suspensions for One Week in a Customized Cold Storage Solution – Preservation of Cell Attachment and MetabolismPless-Petig, G. et al. PlosOne 2012, 7, e40444.HepatocytesLiverRodentCollagenase NB 450 PZ U/L
Comparison of methods for isolating primary hepatocytes from mini pigsLi, Y. et al. Xenotransplantation2016, 0, 1-7.HepatocytesLiverPorcineCollagenase NB 4, Collagenase NB 8

120 PZ U/L, 35 min, 37 ⁰C

Publications on Human Embryonic Stem Cells (hESC)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Good manufacturing practice and clinical-grade human embryonic stem cell linesUnger, C. Human Mol. Gen. 2008, 17, R48-R53.Human embryonic stem cells (hESC)n.a.Humann.a.n.a.
Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studiesHentze, H. et al. Stem Cell Res. 2009, 2, 198-210.Human embryonic stem cells (hESC)Blastocyst-stage preimplantation embryosHumanCollagenase NB 6

1.25 mg/m, 4 min

Publications on Human Umbilical Vein Endothelial Cells (HUVEC)

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banksPolchow, B. et al. J Transl Med. 2012, 10, 98.Human umbilical vein endothelial cells (HUVEC)Umbilical cordHumanCollagenase NB 6

0.12 PZ U/ml, 20 min

Publications on Islets of Langerhans

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Tyrosine hydroxylase activity in the endocrine pancreas: changes induced by short-term dietary manipulationBorelli, M. I. et al. BMC Endocr Disord. 2003, 3.Islets of LangerhansPancreasRodentn.a.n.a.
Influence of Collagenase Loading on Long-Term Preservation of Pig Pancreas by the Two-Layer Method for Subsequent Islet IsolationBrandhorst, D. et al. Transplant. 2005, 79, 38-43.Islets of LangerhansPancreasPorcineCollagenase NB 8, Neutral Protease NBn.a.
Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.Brandhorst, D. et al. Xenotransplant. 2006, 13, 465-470.Islets of LangerhansPancreasPorcine Collagenase NB 8, Neutral Protease NBn.a.
Quantification of Basal and Stimulated ROS Levels as Predictors of Islet Potency and FunctionArmann, B et al. Am J Transplant. 2007, 7, 38-47.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.a.
The Importance of Tryptic-like Activity in Purified Enzyme Blends for Efficient Islet IsolationBrandhorst, H. et al. Transplant. 2009, 87, 370-375.Islets of LangerhansPancreas Rodent and Human Collagenase NB 1, Neutral Protease NBn.a.
Human Islet Isolation for Autologous Transplantation: Comparison of Yield and Function Using SERVA/Nordmark Versus Roche EnzymesAnazawa,T. et al. Am J Transplant. 2009, 9, 2383-2391.Islets of LangerhansPancreas Human Collagenase NB 1, Neutral Protease NBn.a.
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic TissueQi, M. J Vis Exp. 2009, 26, 1125-1126.Islets of LangerhansPancreasHumann.a.n.a.
Large-scale Comparison of Libearse HI and Collagenase NB 1 utilized for Himan Islet IsolationBrandhorst,H. et al. Cell Transplant. 2010, 19, 3-8.Islets of LangerhansPancreas Human Collagenase NB 1, Neutral Protease NBn.a.
Impact of the number of infusions on 2-year results of islet-after-kidney transplantation in the GRAGIL networkBorot,S et al. Transplant. 2011, 92, 1031-1038.Islets of LangerhansPancreas HumanCollagenase NB 1n.a.
Improvement of collagenase distribution with the ductal preservation for human islet isolationShimoda, M. et al. Islets 2012, 4, 130-137.Islets of LangerhansPancreasHumann.a.n.a.
Isolation of human islets for autologous islet transplantation in children and adolescents with chronic pancreatitisBottino,R et al. J Transplant. 2012, 642787.Islets of LangerhansPancreas Humann.a.n.a.
Quality of Isolated Pig Islets Is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe ModelBrandhorst, H. et al. Cell Transplant. 2013, 22, 1477-1483.Islets of LangerhansPancreas Porcine Collagenase NB 1, Neutral Protease NBn.a.
Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociationO'Gorman, D. et al. Islets, 2013, 5, 111-115.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NB High Activen.a.
Human islet cell isolation: the initial step in an islet transplanting program in Shiraz, Southern IranAzarpira,N. et al. Exp Clin Transplant.2014, 12, 139-142.Islets of LangerhansPancreas HumanCollagenase NB 1n.a.
Autologous Islet Transplantation With Remote Islet Isolation After Pancreas Resection for Chronic PancreatitisTai, D.S. et al. JAMA Surg. 2015, 150, 118-124.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.a.
Effects of Donor-, Pancreas-, and Isolation-Related Variables on Human Islet Isolation Outcome: A Systematic ReviewHilling, D. et al. Cell Transplant. 2014, 23, 921-928.Islets of LangerhansPancreasHumanCollagenase NB 1n.a.
Islet Product Characteristics and Factors Related to Successful Human Islet Transplantation From the Collaborative Islet Transplant Registry (CITR) 1999–2010Balamurugan, A.N. et al. Am J Transplant 2014, 14, 2595-2606.Islets of LangerhansPancreasHumanCollagenase NB 1n.a.
National Institutes of Health–Sponsored Clinical Islet Transplantation Consortium Phase 3 Trial: Manufacture of a Complex Cellular Product at Eight Processing FacilitiesRicordi, C. et al. Diabetes 2016, 65, 3418-3428.Islets of LangerhansPancreasHuman

Collagenase NB 1,
Neutral Protease

n.a.
Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?Brandhorst, D. et al. Rev Diabet Stud, 2016, 14, 22-38.Islets of LangerhansPancreasHumann.a.n.a.
Quantifying the Effects of Different Neutral Proteases on Human Islet IntegrityBrandhorst, H. et al. Cell Transplant 2017, 26, 1733-1741.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease, Clostripainn.a.
Evaluation of collagenase gold plus BP protease in isolating islets from human pancreataKhiatah, B. Islets 2018, 4, 51-59.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease2542 PZ U/ml; 266 PZ U/ml
Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet IsolationLoganathan, G. et al. OBM Transplantation 2019, 3.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.a.
Hypoxia-Preconditioned Wharton’s Jelly-Derived Mesenchymal
Stem Cells Mitigate Stress-Induced Apoptosis and Ameliorate Human Islet Survival and Function in Direct Contact Coculture System
Keshtkar, S. et al. StemCell Int. 2020.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Proteasen.a.

Publications on Mesenchymal Stem Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo

Aurich, H. et al. Gut 2009, 58, 570-581.

Mesenchymal Stem CellsAdipose TissueHumanCollagenase NB 4G1 mg/ml (w/w), 37 ⁰C, 30 min
Nonexpanded mesenchymal stem cells for regenerative medicine - yield in stromal vascular fraction from adipose-tissueFaustini, M. et al. Tissue Engin. 2010, 16, 1515-1521.Mesenchymal Stem CellsAdipose TissueHumann.a.n.a.
A new standardized clinical-grade protocol for banking human umbilical cord tissue cellsFazzina, R. et al. Transfusion 2015, 00, 1-10.Mesenchymal Stem CellsUmbilical CordHumanCollagenase NB 61 IU/ml, 
together with 1 IU/ml hyaluronidase, 2.5 hours, 37 ⁰C

Publications on Neural Crest-derived Stem Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeuticsGreiner, J. et al. Eur. Cells Mat. 2011, 22, 403-419.Neural crest-derived stem cellsNasal inferior turbinatesHumanCollagenase NB 41.17 PZ U/ml, 1.5 h, 37 ⁰C
Culture bag systems for clinical applications of adult human neural crest-derived stem cellsGreiner, J. et al. Stem Cell Res Ther. 2014, 5, 34.Neural crest-derived stem cellsNasal inferior turbinatesHumanCollagenase NB 41.17 PZ U/ml, 2 h, 37 ⁰C

Publications on Stem Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
The use of glandular-derived stem cells to improve vascularization in scaffold-mediated dermal regenerationEgana, J. T. et al. Biomterials 2009, 30, 5918-5926.Stem cellsPancreas, Submandibular GlandsMouseCollagenase NB 8n.a.
Glandular Stem Cells: A New Source for Myocardial Repair?Guldner, N. W. et al. Chapter in Reg. Medicine and Tiss. Eng. - Cells and Biomat. 2011 Stem cellsPancreasHumann.a.0.63 PZ U/ml
Cellular modulation of polymeric device surfaces - promise of adult stem cells for neuro-prostheticsRichter, A. et al. Front Neurosci. 2011, 5, 1-10.Stem cellsPancreasRodentn.a.0.63 PZ U/ml

Publications on Stromal Vascular Fraction

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Validation of an Automated Procedure to Isolate Human Adipose Tissue–Derived Cells by Using the Sepax  TechnologyGüven, S. et al. Tissue Engin. 2012, 18, 575-582.Stromal Vascular FractionAdipose TissueHumanCollagenase NB 60.12 PZ U/ml, 1 h, 37 ⁰C
Development and Validation of a Fully GMP-Compliant Process for Manufacturing Stromal Vascular Fraction - A Cost-Effective Alternative to Automated MethodsFrancois, P. Cells 2020, 9, 2158-2182.Stromal Vascular FractionAdipose TissueHumanCollagenase NB 60.25 PZ U/ml, 45 min, 37 ⁰C

Publications on Tumor Cells

TitleReference Cell TypeTissueSpeciesCollagenase ProductExperimental Conditions
Phenotypic heterogeneity among tumorigenic melanoma cells from patients that is reversible and not hierarchically organizedQuintana, E. et al. Cancer Cell 2010, 5, 510-523.Tumor cellsMelanomaHumanCollagenase NB 65 ug/ml, 3 h, 37 ⁰C
A non‑aggressive, highly efficient, enzymatic method for dissociation of human brain‑tumors and brain‑tissues to viable single‑cellsVolovitz, I. et al. BMC Neurosci. 2016, 17,1-10.Tumor cellsBrainHumanNeutral Protease NB 0.11 DMC U/ml

Nordmark Biochemicals' Publication List

TitleReference Cell TypeTissueSpeciesEnzymes
Comparison of Islet Characterization from Use of Standard Crude Collagenase to GMP-Grade Collagenase Enzyme Blends in Preweaned Porcine Islet IsolationsCorrales, N. CellTransplant. 2020, 29, 1-11.           Islets of LangerhansPancreasPorcine

Collagenase AF-1, Collagenase NB 6Neutral Protease AF

Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet IsolationBrandhorst, H. Cell Transplant. 2019, 28, 176-184.Islets of LangerhansPancreasHuman

Collagenase NB 1, Neutral Protease NB, Clostripain

Multicentre Assessment Of Animal-Free Collagenase Af-1 For Human Islet IsolationBrandhorst, D. Cell Transplant. 2017, 26, 1688-1693.Islets of LangerhansPancreasHumanCollagenase AF-1, Neutral Protease AF
Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet IsolationBrandhorst, H. Transplant Direct, 2016, 2, e47.Islets of LangerhansPancreasHumanCollagenase NB 1, Neutral Protease NB, Clostripain
The effect of truncated collagenase class I isomers on human islet isolation outcomeBrandhorst, H. Transplantation 2010, 90, 334-335.Islets of LangerhansPancreasHumanCollagenase NB 1
Degraded collagenase deteriorates islet viabilityBrandhorst, D. Transplant. Proc. 2008, 40, 370-371.Islets of LangerhansPancreasRodent Collagenase NB 1
The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreasBrandhorst, H. Transplantation 2008, 85, 456-461.Islets of LangerhansPancreasRodent Collagenase NB 1
Adjustment of the ratio between collagenase class II and I improves islet isolation outcomeBrandhorst, D. Transplant. Proc. 2005, 37, 3450-3451.Islets of LangerhansPancreasRodent Collagenase NB 1
Assessment of a novel two-component enzyme preparation for human islet isolation and transplantationBucher, P. Transplanation, 2005, 79, 91-97.Islets of LangerhansPancreasHumanCollagenase NB 1
The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreasBrandhorst, H. Transplant Prod. 2005, 37, 215-216.Islets of LangerhansPancreasRodent Collagenase NB 1
Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolationBucher, P. Transplant Proc.2004, 36, 1145-1146.Islets of LangerhansPancreasRodent Collagenase NB 1
Serva collagenase NB1: a new enzyme preparation for human islet isolationBucher, P. Transplant Proc. 2004, 36, 1143-1144.Islets of LangerhansPancreasHumanCollagenase NB 1