3d rendered medically accurate illustration of intestinal villi

Isolation Of Cells from Gastric & Intestine Tissue with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of epithelial cells from gastric mucosa and mastocytes from human intestinal mucosa are presented.

Gastric & Intestine

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
 RodentResearchCollagenase NB 4G Proved Grade0.1 - 0.2 PZ U/ml, 
30 - 60 min, 37 °C
HumanResearch+Collagenase NB 4G Proved Grade0.5 PZ U/ml,
1 h, 37 °C

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
+For further information on GMP grade Collagenase NB please contact us!

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Brief Product Information

Collagenase NB 4G Proved Grade 
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!