Isolation Of Cells from Gastric & Intestine Tissue with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of epithelial cells from gastric mucosa and mastocytes from human intestinal mucosa are presented.
Gastric & Intestine
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4G Proved Grade | 0.1 - 0.2 PZ U/ml, 30 - 60 min, 37 °C | Details |
| Human | Research+ | Collagenase NB 4G Proved Grade | 0.5 PZ U/ml, 1 h, 37 °C | Details |
* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
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Brief Product Information
Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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