Futuristic glowing low polygonal human thyroid gland isolated on dark blue background.

Isolation of Thyroid Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of epithelial cells from human thyroid glands and human thyroid tumor tissue are presented.

Epithelia cells from human thyroid glands

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4G Proved Grade0.25 - 0.3 PZ U/ml, 
RT
Details

 Tumor cells from human thyroid glands

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
Details

+For information on GMP grade Collagenase NB please contact us!

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4 Standard Grade 
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!