Isolation of Thyroid Cells with Collagenase NB
In the following, application details for isolation of epithelial cells from human thyroid glands and human thyroid tumor tissue are presented.
Epithelial Cells from Human Thyroid Glands
|Collagenase NB 4G Proved Grade
|0.25 - 0.3 PZ U/ml,
Tumor Cells from Human Thyroid Glands
|Collagenase NB 8 Broad Range
|3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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