Isolation from Umbilical Cord Tissue with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of, mesenchymal stem cells, human endothelial cells from foreskin (HDMEC) and stem cells from human Wharton’s jelly and human or murine adipose tissue are presented
Mesenchymal Stem Cells from Umbilical Cord
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 6 GMP Grade | 1 IU/ml with 1 IU/ml hyaluronidase, 2.5 hours, 37 ⁰C | Publication |
Endothelial Cells from Umbilical Vein
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human (HUVEC) | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HUVEC) | Clinc | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
Stem Cells from Human Whartons's Jelly
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C | |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C |
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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