Fetus inside the womb

Isolation of Umbilical Cord Tissue with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of human foreskin (HDMEC) and stem cells from human Wharton’s jelly and human or murine adipose tissue are presented

Endothelia cells from umbilical vein

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Human
(HUVEC)
ResearchCollagenase NB 4 Standard Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
Human 
(HUVEC)
ClincCollagenase NB 6 GMP Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details

 Stem cells from human Whartons's jelly

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.13 - 0.15 PZ U/ml,
1 - 6 h , 37 °C
 
HumanClinicCollagenase NB 6 GMP Grade0.13 - 0.15 PZ U/ml,
1 - 6 h , 37 °C
 

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 4 Standard Grade 
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!