Protocol Recommendations
In the following, application details for isolation of, mesenchymal stem cells, human endothelial cells from foreskin (HDMEC) and stem cells from human Wharton’s jelly and human or murine adipose tissue are presented
Mesenchymal Stem Cells from Umbilical Cord
Species | Application Field | Enzymes | Experimental Conditions | Downloads |
---|---|---|---|---|
Human | Clinic | Collagenase NB 6 GMP Grade | 1 IU/ml with 1 IU/ml hyaluronidase, 2.5 hours, 37 ⁰C | Publication |
Endothelial Cells from Umbilical Vein
Species | Application Field | Enzymes | Experimental Conditions | Downloads |
---|---|---|---|---|
Human (HUVEC) | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
Human (HUVEC) | Clinc | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
Stem Cells from Human Whartons's Jelly
Species | Application Field | Enzymes | Experimental Conditions | Downloads |
---|---|---|---|---|
Human | Research | Collagenase NB 4 Standard Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C | |
Human | Clinic | Collagenase NB 6 GMP Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C |

Help us Expand our Knowledge!
If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now!
Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!