For Pancreatic Cell Isolation and other GMP Applications
Collagenase NB 1 GMP Grade is a highly purified collagenase grade. This product is used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB GMP Grade, Collagenase NB 1 GMP Grade is especially applicable to isolation of pancreatic islets of Langerhans. Collagenase NB 1 GMP Grade is recommended for applications like tissue engineering and transplantation into humans which have to meet GMP requirements.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Collagenase class l and class ll1 |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | Manufactured according to international GMP guidelines |
| Microbiological examination | Endotoxin* ≤ 10 EU/mg Absence of Clostridia TAMC (Total Aerobic Microbial Count) ≤ 10/Vial TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial |
| Activities | Collagenase ≥ 2000 U/Vial (PZ** activity according to Wünsch, 25 °C) Collagenase ≥ 3.000 U/mg lyophilisate (PZ**activity according to Wünsch, 25 °C) Neutral Protease ≤ 0.05 U/mg lyophilisate (DMC assay***) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For clinical applications |
Unit definition:
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
**PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
***Neutral Protease:
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS.3
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Related Products
Collagenase NB 1 GMP Grade is often used in combination with Neutral Protease NB GMP Grade. Nordmark also offers Collagenase AF-1 GMP Grade and Neutral Protease AF GMP Grade which are completely free of any animal-based components, excluding the risk of transmission of potential animal-derived pathogens. For research purposes, Nordmark also provides the research grade alternatives Collagenase NB 1 Premium Grade and Neutral Protease NB.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase NB 1 GMP Grade | N0002937 | ≥ 2000 U* | Inquire | Webshoplink |
Documentation
SDS NB 1 GMP 107 KB
CoO NB 1 GMP Grade934 KB
Please contact us for the Certificate of Analysis!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
