Collagenase NB 1 GMP Grade- For Islet Isolation
For Pancreatic Cell Isolation and other GMP Applications
Collagenase NB 1 GMP Grade is a highly purified collagenase grade. This product is used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB GMP Grade, Collagenase NB 1 GMP Grade is especially applicable to isolation of pancreatic islets of Langerhans. Collagenase NB 1 GMP Grade is recommended for applications like tissue engineering and transplantation into humans which have to meet GMP requirements.
|Composition||Collagenase class l and class ll1|
|Molecular weight||70 – 120 kDa (collagenases)|
|Special features||Manufactured according to international GMP guidelines|
Sterility (Ph. Eur.)
Absence of Clostridia
TAMC (Total Aerobic Microbial Count) ≤ 10/Vial
TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial
|Activities||Collagenase ≥ 2000 U/Vial (PZ** activity according to Wünsch, 25 °C)|
Collagenase ≥ 3.000 U/mg lyophilisate (PZ**activity according to Wünsch, 25 °C)
Neutral Protease ≤ 0.05 U/mg lyophilisate (DMC assay***)
|Storage conditions||+2 °C to +8 °C|
|Shipping conditions||Cooled on blue or dry ice depending on the destination|
|Intended use||For clinical applications|
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS.3
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Collagenase NB 1 GMP Grade is often used in combination with Neutral Protease NB GMP Grade. Nordmark also offers Collagenase AF-1 GMP Grade and Neutral Protease AF GMP Grade which are completely free of any animal-based components, excluding the risk of transmission of potential animal-derived pathogens. For research purposes, Nordmark also provides the research grade alternatives Collagenase NB 1 Premium Grade and Neutral Protease NB.
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 188.8.131.52 • CAS [9001-12-1]