Microscopic image of Islet Cells

Collagenase NB 1 Premium Grade-With High Activity

For Cell Isolation from Pancreas, other Organs and Tissues

Collagenase NB 1 Premium Grade is a highly purified collagenase grade used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB Collagenase NB 1 Premium Grade is especially applicable for isolation of pancreatic islets of Langerhans from different species like human or pig. For research use only. If cells are intended for transplantation into humans, Collagenase NB 1 GMP Grade is recommended.

Product Information

OriginClostridium histolyticum
CompositionContains chromatographically highly purified class I and class II collagenase1
Largely free from clostripain, trypsin-like protease and neutral protease
Molecular weight70 – 120 kDa (collagenases)
Special features-
Microbiological examinationEndotoxin*: ≤ 10.0 EU/mg 
ActivitiesCollagenase activity: ≥ 3.00 U/mg lyophilisate (PZ activity** acc. to Wünsch, 25 °C)
Storage conditions+2 °C to +8 °C
Shipping conditionsCooled on blue or dry ice depending on the destination
Intended useFor research use only

Unit definition: 

*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

**PZ Unit (acc. to Wünsch2): 
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:

  1.  Bond, M.D. & van Wart, H.E. (1984) Biochemistry 233077-3091
  2.  Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Related Products

Collagenase NB 1 Premium Grade is often used in combination with Neutral Protease NB. If the application demands GMP requirements, Nordmark offers Collagenase NB 1 GMP Grade that can be used in combination with Neutral Protease NB GMP Grade. Nordmark also offers animal-free products, Collagenase AF-1 GMP Grade and Neutral Protease AF GMP Grade, which are completely free of any animal-based components, thereby excluding the risk of transmission of potential animal-derived pathogens.

Ordering Information

Product NameCat. No. SizePrice
Collagenase NB 1 Premium GradeS1745503≥ 2000 PZ U*Inquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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