Flourescent image of hepatocytes

Collagenase NB 4G Proved Grade-Extra Side Activities

For Isolation of Hepatocytes, Fibroblasts and Epithelial Cells

Collagenase NB 4G Proved Grade is an enzyme mixture which contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The ratio of these activities leads to gentle and efficient tissue dissociation. The product is suitable for isolation of a broad variety of cell types, especially for fibroblasts, human and rodent hepatocytes, frog oocytes and epithelial cells.

Product Information

OriginClostridium histolyticum
Composition      Contains class I and class II collagenase1 and a balanced mix of neutral protease, clostripain and trypsin-like activities
Molecular weight                 70 – 120 kDa (collagenases)
Special features      -
Microbiological examination               -
ActivitiesCollagenase activity: ≥ 0.18 U/mg (PZ activity* acc. to Wünsch, 25 °C)
Storage conditions+2 °C to +8 °C
Shipping conditionsAt ambient temperature worldwide
Intended useFor research use only

*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:

  1.  Bond, M.D. & van Wart, H.E. (1984) Biochemistry 233077-3091
  2.  Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Related Products

If you are dealing with poor cell viability, choose a product with less proteolytic activity like Collagenase NB 4 Standard Grade or lower side activities like Collagenase NB 7D Gentle Grade or Collagenase NB 8 Broad Range.

Ordering Information

Product NameCat. No. SizePrice
Collagenase NB 4G Proved GradeS1746501500 mgInquire
Collagenase NB 4G Proved GradeS1746502 1 gInquire
Collagenase NB 4G Proved GradeS17465035 mgInquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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