3D render Red blood cells transport oxygen to your body's organs and tissues. 

Isolation of Leukocytes with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of leukocytes from murine skin are presented.


SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
  MurineResearchCollagenase NB 7D Gentle Grade0.18 - 0.22 PZ U/ml,
30 min, 37 °C
Contact us

Help us Expand our Knowledge!

If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now! 

Brief Product Information

Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!