Isolation of Leukocytes with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of leukocytes from murine skin are presented.
Leukocytes
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Murine | Research | Collagenase NB 7D Gentle Grade | 0.18 - 0.22 PZ U/ml, 30 min, 37 °C | Contact us |
Help us Expand our Knowledge!
If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now!
Brief Product Information
Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!