3D Model of skin cancer cells

Isolation of Melanoma Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of melanoma cells from murine skin are presented.

Melanoma Cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
ResearchCollagenase NB 8 Broad Range0.12 PZ U/ml,
30 min, 37 °C

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Brief Product Information

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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