Isolation of Osteoblasts with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of osteoblasts from murine and human bone tissue are presented.
Osteoblasts
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range & Neutral Protease NB | 1.5 - 2 PZ U/ml (NB 8), 0.25 DMC U/ml (NP), 2 - 4 h , 37 °C | Details |
| Mouse | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 5 - 10 min, 37 °C** | Details |
**0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
+4-5 consecutive digestion steps are recommended
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Brief Product Information
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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