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Isolation of Osteoblasts with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of osteoblasts from murine and human bone tissue are presented.

Osteoblasts

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
 HumanResearch+Collagenase NB 8 Broad Range &
Neutral Protease NB
 
1.5 - 2 PZ U/ml (NB 8), 
0.25 DMC U/ml (NP),
2 - 4 h , 37 °C 
Details
MouseResearchCollagenase NB 4 Standard Grade 0.15 - 0.25 PZ U/ml, 
5 - 10 min, 37 °C**
Details​​​​​​​

**0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
+4-5 consecutive digestion steps are recommended

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Brief Product Information

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4 Standard Grade 
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!