Neutral Protease NB GMP Grade - For the Extra Boost
Alone or in Combination with other GMP Collagenases
Neutral Protease is a metalloprotease that can cleave peptide bonds in proteins such as collagen, proteoglycan, fibronectin, elastin etc. Due to these properties, it is suitable for the isolation of a broad range of cell types by tissue dissociation. In combination with Collagenase NB 1 GMP Grade, Neutral Protease NB GMP Grade is especially suited for isolation of islets of Langerhans from human pancreas. However, Neutral Protease NB GMP Grade can also be used in combination with Collagenase NB 6 GMP Grade for applications like tissue engineering and transplantation into humans which have to meet GMP requirements .
|Composition||Contains chromatographically purified neutral protease|
|Molecular weight||34 kDa|
|Special features||Manufactured according to international GMP guidelines|
Sterility (Ph. Eur.)
Absence of Clostridia
TAMC (Total Aerobic Microbial Count) ≤ 10/Vial
TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial
|Activities||Neutral Protease ≥ 100 U/Vial (DMC assay**)|
Neutral Protease ≥ 0.50 U/mg lyophilisate (DMC assay**)
|Storage conditions||+2 °C to +8 °C|
|Shipping conditions||Cooled on blue or dry ice depending on the destination|
|Intended use||For clinical applications|
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.
- Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
For cell isolation, Neutral Protease NB GMP Grade is often used in combination with Collagenase NB 1 GMP Grade. Nordmark also offers animal-free products, Neutral Protease AF GMP Grade and Collagenase AF-1 GMP Grade, which are completely free of any animal-based components, excluding the risk of transmission of potential animal-derived pathogens. For research purposes, Nordmark provides the research grade alternatives Neutral Protease NBand Collagenase NB 1 Premium Grade.
|Product Name||Cat. No.||Size||Contact us||Order|
|Neutral Protease NB GMP Grade||N0002936||≥ 100 DMC*||Inquire||Webshoplink|
- SDS Neutral Protease GMP107 KB
Please contact us for the Certificate of Analysis!
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-642-4 • WGK: 1 • HS: 35079090 • EC 220.127.116.11 • CAS [9001-92-7]