Isolation from Reproductive Tissues with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of xenopus oocytes, isolation of cells from human endometrium, human foreskin (HDMEC) and umbilical cord (HUVEC), epithelial cells from murine mammary glands and breast or uterus tumor cells are presented.
Oocytes from Xenopus Laevis
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Frog | Research | Collagenase NB 4G Proved Grade | 0.35 - 0.5 PZ U/ml, 1.5 - 2 h, RT | Details |
Amnion and Chorion Cells from Human Fetal Tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Details |
Endometrial Cells from Human Cervical Tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml, 30 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.2 - 0.3 PZ U/ml, 30 min, 37 °C | Details |
Endothelia Cells from Human Foreskin and Umbilical Vein
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human (HDMEC) | Research | Collagenase NB 4 Standard Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HDMEC) | Clinic | Collagenase NB 6 GMP Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HUVEC) | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HUVEC) | Clinic | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
Epithelial Cells from Murine Mammary Gland
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 4 Standard Grade | 0.34 PZ U/ml, 15 - 25 min, 37 °C | Details |
Tumor Cells from Breast and Uterus
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Contact us |
+For information on GMP grade Collagenase NB please contact us!
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!