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Isolation of Reproductive Tissues with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of Xenopus Oocytes, isolation of cells from human endometrium, human foreskin (HDMEC) and umbilical cord (HUVEC), epithelial cells from murine mammary glands and breast or uterus tumor cells are presented.

Oocytes from Xenopus Laevis

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
FrogResearchCollagenase NB 4G Proved Grade0.35 - 0.5 PZ U/ml,
1.5 - 2 h, RT
Details

 Amnion and chorion cells from human fetal tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
Details

Endometrial cells from human cervical tissue 

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml, 
30 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml, 
30 min, 37 °C
Details

Endothelia cells from human foreskin and umbilical vein 

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Human
(HDMEC)
ResearchCollagenase NB 4 Standard Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
Human
(HDMEC)
ClinicCollagenase NB 6 GMP Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
Human
(HUVEC)
ResearchCollagenase NB 4 Standard Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
Human
(HUVEC)
ClinicCollagenase NB 6 GMP Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details

 Epithelia cells from murine mammary gland

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 4 Standard Grade0.34 PZ U/ml, 
15 - 25 min, 37 °C
Details

 Tumor cells from breast and uterus

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
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Brief Product Information

Collagenase NB 6 GMP Grade 
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 4 Standard Grade 
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion please contact us!