Pancreas

Dissociation of Pancreatic Tissue with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of human acinar cells and islets of Langerhans from human, rodent and porcine pancreatic tissue are presented.

Acinar Cells from Pancreatic Tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range0.05 - 0.1 PZ U/ml, 
1 h, 37 °C
Details

 Islet of Langerhans from Pancreatic Tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 1 Premium Grade &
Neutral Protease NB
Per gram tissue:
20 PZ U (NB 1),
0.8 - 1.5 DMC U (NP),
15 - 30 min, 37 °C
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HumanClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade
Per gram tissue:
20 PZ U (NB 1),
0.8 - 1.5 DMC U (NP),
15 - 30 min, 37 °C
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Porcine (adult)ResearchCollagenase NB 8 Broad Range &
Neutral Protease NB
Per gram tissue:
4.4 PZ U (NB 8),
0.5 - 0.7 DMC U (NP),
37 °C
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Porcine (adult)ClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade

 

Per gram tissue:
15 - 18  PZ U (NB 1),
0.8 - 1.3 DMC U (NP),
15 - 30 min, 37 °C
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Porcine (neonatal)ResearchCollagenase NB 4 Standard Grade0.17 - 0.2 PZ U/ml,
10 - 20 min, 37 °C
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Porcine (neonatal)ClinicCollagenase NB 6 GMP Grade0.17 - 0.2 PZ U/ml,
10 - 20 min, 37 °C
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RodentResearchCollagenase NB 8 Broad Range1.5 - 1.7 PZ U/ml
14 - 17 min, 37 °C
Details

+For information on GMP grade Collagenase NB please contact us!

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 1 Premium Grade 
This product is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade.

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4 Standard Grade 
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!