Squamous epithelial cells under microscope view for education histology

Isolation of Epithelial Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of epithelial cells from murine mammary glands, rodent gastric mucosa and human thyroid glands are presented.

Epithelial Cells

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 4G Proved Grade 0.25 - 0.3 PZ U/ml, 
MouseResearch+Collagenase NB 4 Standard Grade  0.34 PZ U/ml, 
15 - 25 min, 37 °C
RodentResearch+Collagenase NB 4G Proved Grade 0.1 - 0.2 PZ U/ml, 
30 - 60 min, 37 °C

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Brief Product Information

Collagenase NB 4G Proved Grade
This product has an activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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