Pigs

Porcine Applications

Protocol Recommendations

In the following, application details for cell isolation from porcine tissue are presented.

Liver

Hepatocytes from liver tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
PigResearchCollagenase NB 8 Broad Range0.2 - 0.3 PZ U/mlContact us

Pancreas

 Islet of Langerhans from pancreatic tissue 

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Porcine (adult)ResearchCollagenase NB 8 Broad Range &
Neutral Protease NB
Per gram tissue:
4.4 PZ U (NB 8),
0.5 - 0.7 DMC U (NP),
37 °C
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Porcine (adult)ClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade

 

Per gram tissue:
15 - 18  PZ U (NB 1),
0.8 - 1.3 DMC U (NP),
15 - 30 min, 37 °C
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Porcine (neonatal)ResearchCollagenase NB 4 Standard Grade0.17 - 0.2 PZ U/ml,
10 - 20 min, 37 °C
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Porcine (neonatal)ClinicCollagenase NB 6 GMP Grade0.17 - 0.2 PZ U/ml,
10 - 20 min, 37 °C
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Brief Product Information

Collagenase NB 6 GMP Grade:  
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 1 Premium Grade
This product is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade.

Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade.

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4 Standard Grade  
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us