3D model of stem cells

Collagenase NB 5 Sterile Grade-For Process Development

For Human ADSCs, Chondrocytes and Stem Cell Passaging

Collagenase NB 5 Sterile Grade is a natural enzyme mixture that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease and is sterile according to European Pharmacopoeia. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. Collagenase NB 5 is applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and their passaging, and rodent osteoblasts. This product is also part of our Translational Collagenase NB portfolio. It has enzymatic properties similiar to Collagenase NB 4 Standard Grade for use in research applications and Collagenase NB 6 GMP Grade for clinical applications like regenerative medicine and transplantation.

Product Information

OriginClostridium histolyticum
CompositionContains class I and class II collagenase1 and a balanced ratio of proteolytic side activities
Molecular weight70 – 120 kDa (collagenases)
Special features-
Microbiological examination-
ActivitiesCollagenase activity: ≥ 0.10 U/mg (PZ activity* acc. to Wünsch, 25 °C)
Storage conditions+2 °C to +8 °C
Shipping conditionsAt ambient temperature worldwide
Intended useFor research use only

*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:

  1.  Bond, M.D. & van Wart, H.E. (1984) Biochemistry 233077-3091
  2.  Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Related Products

For initial testing and protocol optimization, Collagenase NB 4 Standard Grade can be used. If the application has to meet GMP requirements, Nordmark also offers Collagenase NB 6 GMP Grade which has comparable enzymatic properties to Collagenase NB 5 Sterile Grade and Collagenase NB 4 Standard Grade.

Ordering Information

Product NameCat. No. SizePrice
Collagenase NB 5 Sterile GradeN00027781 gInquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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