Collagenase NB 5 Sterile Grade-For Process Development
For Human ADSCs, Chondrocytes and Stem Cell Passaging
Collagenase NB 5 Sterile Grade is a natural enzyme mixture that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease and is sterile according to European Pharmacopoeia. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. Collagenase NB 5 is applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and their passaging, and rodent osteoblasts. This product is also part of our Translational Collagenase NB portfolio. It has enzymatic properties similiar to Collagenase NB 4 Standard Grade for use in research applications and Collagenase NB 6 GMP Grade for clinical applications like regenerative medicine and transplantation.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains class I and class II collagenase1 and a balanced ratio of proteolytic side activities |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | - |
| Microbiological examination | - |
| Activities | Collagenase activity: ≥ 0.10 U/mg (PZ activity* acc. to Wünsch, 25 °C) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For research use only |
*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Related Products
For initial testing and protocol optimization, Collagenase NB 4 Standard Grade can be used. If the application has to meet GMP requirements, Nordmark also offers Collagenase NB 6 GMP Grade which has comparable enzymatic properties to Collagenase NB 5 Sterile Grade and Collagenase NB 4 Standard Grade.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase NB 5 Sterile Grade | N0002778 | 1 g | Inquire | Webshoplink |
Documentation
SDS NB 5109 KB
CoO NB 5 Sterile Grade888 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
