Isolation of Neurons with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of neurons from human and rodent dorsal root ganglia are presented.
Neurons
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °C | Contact us |
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 h, 37 °C** | Details |
**4-5 consecutive digestion steps are recommended
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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