3D model of the inside the brain. Concept of neurons and nervous system.

Isolation of Neurons with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of neurons from human and rodent dorsal root ganglia are presented.

Neurons

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °CDetails
HumanClinicCollagenase NB 6 GMP Grade 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °CDetails
RodentResearchCollagenase NB 4 Standard Grade 0.15 - 0.25 PZ U/ml, 
30 - 60 h, 37 °C**
Details

**4-5 consecutive digestion steps are recommended
 

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 4 Standard Grade 
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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