Osteoporosis stage 4 of 4 - upper limb bone - 3d rendering

Isolation of Osteoblasts with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of osteoblasts from murine and human bone tissue are presented.

Bone

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range &
Neutral Protease NB
1.5 - 2 PZ U/ml (NB 8), 
0.25 DMC U/ml (NP),
2 - 4 h, 37 °C 
Details
MouseResearchCollagenase NB 4 Standard Grade0.15 - 0.25 PZ U/ml, 
5 - 10 min, 37 °C**
Details

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
**0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
+For information on GMP grade Collagenase NB please contact us!

Help us Expand our Knowledge!

If you share protocol information (concentration, incubation time and temperature etc.) with us, we will send you a free giant microbe! Write to us now! 

Brief Product Information

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 4 Standard Grade 
This product shows a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Neutral Protease NB  
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please contact us!