Isolation of Osteoblasts with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of osteoblasts from murine and human bone tissue are presented.
Bone
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range & Neutral Protease NB | 1.5 - 2 PZ U/ml (NB 8), 0.25 DMC U/ml (NP), 2 - 4 h, 37 °C | Details |
| Mouse | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 5 - 10 min, 37 °C** | Details |
* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
**0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
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Brief Product Information
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product shows a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
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