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Isolation of Skin Cells with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of endothelial cells from rodent aortas, human foreskin (HDMEC) and for isolation of fibroblasts from rodent and human skin and connective tissue are presented.

Endothelial Cells from Rodent Aortas and Human Foreskin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads

Human
(HDMEC)

ResearchCollagenase NB 4 Standard Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
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Human
(HDMEC)

ClinicCollagenase NB 6 GMP Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
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RodentResearchCollagenase NB 4 Standard Grade0.12 - 0.15 PZ U/ml, 
10 - 20 min, 37 °C
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Melanoma Cells from Murine Skin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MouseResearchCollagenase NB 8 Broad Range0.12 PZ U/ml,
30 min, 37 °C
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 Fibroblasts from Murine and Human Skin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 4G Proved Grade0.26 PZ U/ml,
2 h, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml,
2 h, 37 °C
Details
RodentResearchCollagenase NB 4G Proved Grade0.2 - 0.5 PZ U/ml, 
12 - 20 h, 37 °C
Details

Leukocytes from Murine Skin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MurineResearchCollagenase NB 7D Gentle Grade0.18 - 0.22 PZ U/ml,
30 min, 37 °C
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+For information on GMP grade Collagenase NB please contact us!

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Brief Product Information

Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.

Collagenase NB 4G Proved Grade 
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!