Protocol Recommendations
In the following, application details for isolation of endothelial cells from rodent aortas, human foreskin (HDMEC) and for isolation of fibroblasts from rodent and human skin and connective tissue are presented.
Endothelial Cells from Rodent Aortas and Human Foreskin
Species | Application Field | Enzymes | Experimental Conditions | Downloads |
---|---|---|---|---|
Human (HDMEC)
| Research | Collagenase NB 4 Standard Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Contact us |
Human (HDMEC) | Clinic | Collagenase NB 6 GMP Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Contact us |
Rodent | Research | Collagenase NB 4 Standard Grade | 0.12 - 0.15 PZ U/ml, 10 - 20 min, 37 °C | Contact us |
Melanoma Cells from Human & Murine Skin
Species | Application Field | Enzymes | Experimental Conditions | Downloads |
---|---|---|---|---|
Mouse | Research | Collagenase NB 8 Broad Range | 0.12 PZ U/ml, 30 min, 37 °C | Contact us |
Human | Clinic | Collagenase NB 6 GMP Grade | 0.001 PZ U/ml, 3 h, 37°C | Publication |
Fibroblasts from Human & Murine Skin
Species | Application Field | Enzymes | Experimental Conditions | Downloads |
---|---|---|---|---|
Human | Research+ | Collagenase NB 4G Proved Grade | 0.26 PZ U/ml, 2 h, 37 °C | Details |
Human | Clinic | Collagenase NB 6 GMP Grade | 0.2 - 0.3 PZ U/ml, 2 h, 37 °C | Details |
Rodent | Research | Collagenase NB 4G Proved Grade | 0.2 - 0.5 PZ U/ml, 12 - 20 h, 37 °C | Details |
Leukocytes from Murine Skin
Species | Application Field | Enzymes | Experimental Conditions | Downloads |
---|---|---|---|---|
Murine | Research | Collagenase NB 7D Gentle Grade | 0.18 - 0.22 PZ U/ml, 30 min, 37 °C | Contact us |
+For information on GMP grade Collagenase NB please contact us!
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!