Collagenase NB 6 GMP Grade- For Stem Cell Isolation
For Human ADSCs, Chondrocytes and Fibroblasts
Collagenases NB 6 GMP Grade from Clostridium histolyticum is a natural mixture of enzymatic activities and consists of collagenase class I and class II, neutral protease and clostripain. It is used for tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal and endothelial cells. This collagenase is manufactured under highest standards and is ideal for applications which have to meet GMP requirements like tissue engineering and transplantation.
|Composition||Collagenase class I and class II1 as well as proteolytic activities such as neutral protease and clostripain|
|Molecular weight||70 – 120 kDa (collagenases)|
|Special features||Manufactured according to international GMP guidelines|
Sterile according to European Pharmacopoeia
Sterility (Ph. Eur.)
Absence of Clostridia
|Activities||Collagenase activity: ≥ 0.100 PZ U/mg lyophilisate (PZ activity** acc. to Wünsch, 25 °C)|
|Storage conditions||+2 °C to +8 °C|
|Shipping conditions||At ambient temperature worldwide|
|Intended use||For clinical applications|
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Since Collagenase NB 6 GMP Grade is designed for clinical applications under GMP requirements, Nordmark also offers an equivalent product for initial protocol and application development. Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 6 GMP Grade and is a cost effective alternative for protocol optimization. If a sterile product is needed, Collagenase NB 5 Sterile Grade may also be used in order to establish protocols.
|Product Name||Cat. No.||Size||Contact us||Order|
|Collagenase NB 6 GMP Grade||N0002880||100 mg||Inquire||https://pubmed.ncbi.nlm.nih.gov/27226576/|
|Collagenase NB 6 GMP Grade||N0002779||1 g||Inquire||https://ak2elkn1k.accounts.ondemand.com/saml2/idp/sso/ak2elkn1k.accounts.ondemand.com|
|Hazard statements||H315 - H317 - H319 - H334 - H335|
|Reaction||P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364|
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 22.214.171.124 • CAS [9001-12-1]