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Human Applications

Protocol Recommendations

In the following, application details for cell isolation from human tissue are presented.

Adipose/Fat

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml*,
30 - 60 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml*,
30 - 60 min , 37 °C
Details
HumanClinicCollagenase AF-1 GMP Grade &
Neutral Protease AF GMP Grade

0.4 PZ U/ml**& 0.2 DMC U/ml**, 50 min, 37°C

Details​​​​​​​

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue

Bone

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range &
Neutral Protease NB
1.5 - 2 PZ U/ml (NB 8), 
0.25 DMC U/ml (NP),
2 - 4 h, 37 °C 
Details​​​​​​​

+For information on GMP grade Collagenase NB please contact us!

Cartilage

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade 0.3 - 0.4 PZ U/ml, 
4 - 16 h, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade 0.3 - 0.4 PZ U/ml,
4 - 16 h, 37 °C
Details​​​​​​​​​​​​​​

Colon

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+ Collagenase NB 4G Proved Grade 0.5 - 1 PZ U/ml,
RT
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+For information on GMP grade Collagenase NB please contact us!

Gastric/Intestine

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 4G Proved Grade0.5 PZ U/ml,
1 h, 37 °C
Details​​​​​​​

+For further information on GMP grade Collagenase NB please contact us!

Heart

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4G Proved Grade 0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details

Liver

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4G Proved Grade 0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details​​​​​​​

Lung

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
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+For further information on GMP grade Collagenase NB please contact us!

Muscle

Cardiomyocytes from cardiac muscle tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range0.8 - 1.0 PZ U/ml, 
2 - 3.5 h, 37 °C
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 Myocytes from skeletal muscle tissue:

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range0.4 - 0.6 PZ U/ml,
37 °C
Details​​​​​​​

+For information on GMP grade Collagenase NB please contact us!

Neural

Neural

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °CDetails
HumanClinicCollagenase NB 6 GMP Grade 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °CDetails

 Schwann cells from neuronal tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade 
0.5 PZ U/ml, 2 DCM U/ml, 
18 h, 37 °C
Details​​​​​​​​​​​​​​

Pancreas

Acinar cells from pancreatic tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range0.05 - 0.1 PZ U/ml, 
1 h, 37 °C
Details

 Islet of Langerhans from pancreatic tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 1 Premium Grade &
Neutral Protease NB
Per gram tissue:
20 PZ U (NB 1),
0.8 - 1.5 DMC U (NP),
15 - 30 min, 37 °C
 
HumanClinicCollagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade
Per gram tissue:
20 PZ U (NB 1),
0.8 - 1.5 DMC U (NP),
15 - 30 min, 37 °C
 

+For information on GMP grade Collagenase NB please contact us!

Reproductive

Amnion and chorion cells from human fetal tissue

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
Details

Endometrial cells from human cervical tissue 

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.2 - 0.3 PZ U/ml, 
30 min, 37 °C
Details​​​​​​​
HumanClinicCollagenase NB 6 GMP Grade0.2 - 0.3 PZ U/ml, 
30 min, 37 °C
Details​​​​​​​

Endothelia cells from human foreskin and umbilical vein

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Human
(HDMEC)
ResearchCollagenase NB 4 Standard Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
Details​​​​​​​
Human
(HDMEC)
ClinicCollagenase NB 6 GMP Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
Details​​​​​​​
Human
(HUVEC)
ResearchCollagenase NB 4 Standard Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details​​​​​​​
Human
(HUVEC)
ClinicCollagenase NB 6 GMP Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details​​​​​​​

Tumor cells from breast and uterus

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
 

+For information on GMP grade Collagenase NB please contact us!

Skin

Endothelia cells from human foreskin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads

Human
(HDMEC)

ResearchCollagenase NB 4 Standard Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
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Human
(HDMEC)

ClinicCollagenase NB 6 GMP Grade0.5 - 0.7 PZ U/ml, 
0.5 - 2 h, 37 °C
Contact us

 Fibroblasts from human skin

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 4G Proved Grade0.26 PZ U/ml,
2 h, 37 °C
Details

Thyroid

Epithelia cells from human thyroid glands

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.25 - 0.3 PZ U/ml, 
RT
Details
HumanClinicCollagenase NB 6 GMP Grade0.25 - 0.3 PZ U/ml, 
RT
Details

Tumor cells from human thyroid glands

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
Contact us​​​​​​​

 

+For information on GMP grade Collagenase NB please contact us!

Tumor

Tumor cells from human breast, uterus, thyroid gland or lung

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
Details

 Tumor cells from human colon

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 4G Proved Grade0.5 - 1 PZ U/ml,
RT
Details​​​​​​​​​​​​​​

+For information on GMP grade Collagenase NB please contact us!

Umbilical Cord

Endothelia cells from umbilical vein

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
Human
(HUVEC)
ResearchCollagenase NB 4 Standard Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details
Human 
(HUVEC)
ClincCollagenase NB 6 GMP Grade0.15 - 0.25 PZ U/ml, 
0.5 - 2 h, 37 °C
Details

Stem cells from human Whartons's jelly

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4 Standard Grade0.13 - 0.15 PZ U/ml,
1 - 6 h , 37 °C
 
HumanClinicCollagenase NB 6 GMP Grade0.13 - 0.15 PZ U/ml,
1 - 6 h , 37 °C
 

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Brief Product Information

Collagenase NB 6 GMP Grade  
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 1 Premium Grade
This product is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade.

Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade. 

Collagenase AF-1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenase activity of  ≥ 3.00 U/mg.  It is produced under GMP guidelines and completely free of any animal based components.

Neutral Protease AF GMP Grade
This neutral protease is a highly purified enzyme with a proteolytic activity of ≥ 0.50 DMC U/mg. It is produced under GMP guidelines and completely free of any animal based components.

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.

Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 4 Standard Grade  
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us