Human Applications
Protocol Recommendations
In the following, application details for cell isolation from human tissue are presented.
Adipose/Fat
Adipocytes & Adipose-Derived Stem Cells
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Murine | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
| Human | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min , 37 °C | Details |
Stromal Vascular Fraction (Stem Cells)
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase AF-1 GMP Grade & Neutral Protease AF GMP Grade | 0.4 PZ U/ml & 0.2 DMC U/ml**, 50 min, 37 °C | Details & Laboratory Note |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.12 - 0.25 PZ U/ml, 45 - 60 min, 37 °C | Publication 1 & Publication 2 |
* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
Mesenchymal Stem Cells from Adipose Tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 4G Proved Grade | 1 mg/ml (w/w), 37 ⁰C, 30 min | Publication |
Bone
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range & Neutral Protease NB | 1.5 - 2 PZ U/ml (NB 8), 0.25 DMC U/ml (NP), 2 - 4 h, 37 °C | Details |
+For information on GMP grade Collagenase NB please contact us!
Cartilage
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.3 - 0.4 PZ U/ml, 4 - 16 h, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.3 - 0.4 PZ U/ml, 4 - 16 h, 37 °C | Details |
Colon
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 4G Proved Grade | 0.5 - 1 PZ U/ml, RT | Contact us |
+For information on GMP grade Collagenase NB please contact us!
Embryonic Stem Cells
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 6 GMP Grade | 1.25 mg/ml, 4min , 37 °C | Publication |
Gastric/Intestine
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 4G Proved Grade | 0.5 PZ U/ml, 1 h, 37 °C | Details |
+For further information on GMP grade Collagenase NB please contact us!
Heart
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4G Proved Grade | 0.25 - 0.35 PZ U/ml, 10 - 20 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.25 - 0.35 PZ U/ml, 10 - 20 min, 37 °C | Details |
Liver
Hepatocytes
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4G Proved Grade | 0.25 - 0.35 PZ U/ml, 10 - 20 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.25 - 0.35 PZ U/ml, 10 - 20 min, 37 °C | Details |
Human Liver Stem Cells (HLSC)
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 1, Neutral Protease NB | 0.6 mg/ml NB 1, 0.73 mg/ml NP NB, 37 ⁰C | Publication |
Lung
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Contact us |
+For further information on GMP grade Collagenase NB please contact us!
Muscle
Cardiomyocytes from cardiac muscle tissue:
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 0.8 - 1.0 PZ U/ml, 2 - 3.5 h, 37 °C | Contact us |
Myocytes from skeletal muscle tissue:
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 0.4 - 0.6 PZ U/ml, 37 °C | Details |
+For information on GMP grade Collagenase NB please contact us!
Neural
Neural
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °C | Details |
Schwann cells from neuronal tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 1 GMP Grade & Neutral Protease NB GMP Grade | 0.5 PZ U/ml, 2 DCM U/ml, 18 h, 37 °C | Details |
Pancreas
Acinar cells from pancreatic tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 0.05 - 0.1 PZ U/ml, 1 h, 37 °C | Details |
Islet of Langerhans from pancreatic tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 1 Premium Grade & Neutral Protease NB | Per gram tissue: 20 PZ U (NB 1), 0.8 - 1.5 DMC U (NP), 15 - 30 min, 37 °C | |
| Human | Clinic | Collagenase NB 1 GMP Grade & Neutral Protease NB GMP Grade | Per gram tissue: 20 PZ U (NB 1), 0.8 - 1.5 DMC U (NP), 15 - 30 min, 37 °C |
+For information on GMP grade Collagenase NB please contact us!
Reproductive
Amnion and chorion cells from human fetal tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Details |
Endometrial cells from human cervical tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml, 30 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.2 - 0.3 PZ U/ml, 30 min, 37 °C | Details |
Endothelia cells from human foreskin and umbilical vein
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human (HDMEC) | Research | Collagenase NB 4 Standard Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HDMEC) | Clinic | Collagenase NB 6 GMP Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HUVEC) | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HUVEC) | Clinic | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
Tumor cells from breast and uterus
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C |
+For information on GMP grade Collagenase NB please contact us!
Skin
Endothelia cells from human foreskin
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
Human | Research | Collagenase NB 4 Standard Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Contact us |
Human | Clinic | Collagenase NB 6 GMP Grade | 0.5 - 0.7 PZ U/ml, 0.5 - 2 h, 37 °C | Contact us |
Fibroblasts from human skin
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 4G Proved Grade | 0.26 PZ U/ml, 2 h, 37 °C | Details |
Melanoma Cells from Human Skin
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.001 PZ U/ml, 3 h, 37°C | Publication |
Thyroid
Epithelia cells from human thyroid glands
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.25 - 0.3 PZ U/ml, RT | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.25 - 0.3 PZ U/ml, RT | Details |
Tumor cells from human thyroid glands
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Contact us |
+For information on GMP grade Collagenase NB please contact us!
Tumor
Tumor Tissue Human from Brain
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Neutral Protease NB | 0.11 DMC U/ml | Contact us |
Tumor Tissue from Human Breast, Thyroid Gland or Lung
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Details |
Tumor Tissue from Human Colon
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 4G Proved Grade | 0.5 - 1 PZ U/ml, RT | Details |
+For information on GMP grade Collagenase NB please contact us!
Umbilical Cord
Endothelia cells from umbilical vein
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human (HUVEC) | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
| Human (HUVEC) | Clinc | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 0.5 - 2 h, 37 °C | Details |
Stem cells from human Whartons's jelly
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C | |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.13 - 0.15 PZ U/ml, 1 - 6 h , 37 °C |
Mesenchymal Stem Cells from Umbilical Cord
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 6 GMP Grade | 1 IU/ml with 1 IU/ml hyaluronidase, 2.5 hours, 37 ⁰C | Publication |
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 1 Premium Grade
This product is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade.
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade.
Collagenase AF-1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenase activity of ≥ 3.00 U/mg. It is produced under GMP guidelines and completely free of any animal based components.
Neutral Protease AF GMP Grade
This neutral protease is a highly purified enzyme with a proteolytic activity of ≥ 0.50 DMC U/mg. It is produced under GMP guidelines and completely free of any animal based components.
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!