Isolation of Tumor Cells with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of cells from tumor tissue from brain, breast, uterus, thyroid gland, lung, and colon are presented.
Tumor Tissue Human from Brain
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Neutral Protease NB | 0.11 DMC U/ml | Contact us |
Tumor Tissue from Human Breast, Thyroid Gland or Lung
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 8 Broad Range | 3.5 - 5.5 PZ U/ml, 3 - 5 h, 37 °C | Details |
Tumor Tissue from Human Colon
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research+ | Collagenase NB 4G Proved Grade | 0.5 - 1 PZ U/ml, RT | Details |
Tumor Tissue from Murine Lungs
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Murine | Research+ | Collagenase NB 7D Gentle Grade | 0.5 - 0.6 PZ U/ml, 1.5 - 2 h, 37 °C, add 100 μg/mL DNase | Contact us |
Tumor Cells from Human & Murine Skin
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse | Research | Collagenase NB 8 Broad Range | 0.12 PZ U/ml, 30 min, 37 °C | Contact us |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.001 PZ U/ml, 3 h, 37°C | Publication |
+For information on GMP grade Collagenase NB please contact us!
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Brief Product Information
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
Collagenase NB 4G Proved Grade
This product has an activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us!