Isolation of Tumor Cells with Collagenase NB

Protocol Recommendations

 In the following, application details for isolation of cells from tumor tissue from breast, uterus, thyroid gland, lung, and colon are presented.

Tumor Tissue from Human Breast, Thyroid Gland or Lung

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 8 Broad Range3.5 - 5.5 PZ U/ml,
3 - 5 h, 37 °C
Details

Tumor Tissue from Murine Lungs

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
MurineResearchCollagenase NB 7D Gentle Grade0.5 - 0.6 PZ U/ml,
1.5 - 2 h, 37 °C,
add 100 μg/mL DNase

 

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Tumor Tissue from Colon

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearch+Collagenase NB 4G Proved Grade0.5 - 1 PZ U/ml,
RT
Details

+For information on GMP grade Collagenase NB please contact us!

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Brief Product Information

Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Collagenase NB 7D Gentle Grade
This product is characterized by a slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.

Collagenase NB 4G Proved Grade
This product has an activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
For further information on optimization of tissue digestion, please contact us