Neutral Protease NB - Collagenase Companion
For Pancreatic Islet Isolation and Other Dissociation
Neutral Protease NB is a metalloprotease suitable for tissue dissociation applications. This enzyme cuts diverse peptides in front of hydrophobic amino acids and thereby creates smaller fragments. For cell isolation, Neutral Protease NB is often used in combination with purified Collagenase NB 1 Premium Grade as well as Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range. If cells are intended for transplantation into humans, Neutral Protease NB GMP Grade is recommended.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains chromatographically purified neutral protease |
| Molecular weight | 34 kDa |
| Special features | - |
| Microbiological examination | - |
| Activities | Neutral Protease activity: ≥ 0.50 U/mg (DMC assay*) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | Cooled on blue or dry ice depending on the destination |
| Intended use | For research use only |
Unit definition:
*Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.
References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Related Products
Neutral Protease NB is often used in combination with Collagenase NB 1 Premium Grade, but Neutral Protease NB can be combined with other collagenases like Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range as well. For applications with GMP requirements, Nordmark offers Neutral Protease NB GMP Grade, which can be used in combination with Collagenase NB 1 GMP Grade. Along with the Collagenase NB product line, Nordmark also offers animal-free products, Neutral Protease AF GMP Grade and Collagenase AF-1 GMP Grade, which are completely free of any animal-based components, thereby excluding the risk of transmission of potential animal-derived pathogens.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Neutral Protease NB | S3030111 | 50 DMC* U | Inquire | Webshoplink |
| Neutral Protease NB | S3030112 | 100 DMC* U | Inquire | Webshoplink |
Documentation
SDS Neutral Protease NB109 KB
CoO Neutrale Protease890 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-642-4 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-92-7]
