Micoskpic Image of isolated Islets

Neutral Protease NB - Collagenase Companion

For Pancreatic Islet Isolation and Other Dissociation

Neutral Protease NB is a metalloprotease suitable for tissue dissociation applications. This enyzme cuts diverse peptides before hydrophobic amino acids and thereby creates smaller fragments. For cell isolation, Neutral Protease NB is often used in combination with purified Collagenase NB 1 Premium Grade as well as Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range.  If cells are intended for transplantation into humans, Neutral Protease NB GMP Grade is recommended.

Product Information

OriginClostridium histolyticum
CompositionContains chromatographically purified neutral protease
Molecular weight34 kDa
Special features-
Microbiological examination-
ActivitiesNeutral Protease activity: ≥ 0.50 U/mg (DMC assay*)
Storage conditions+2 °C to +8 °C
Shipping conditionsCooled on blue or dry ice depending on the destination
Intended useFor research use only

Unit definition: 
*Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS1.
 

References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Related Products

Neutral Protease NB is often used in combination with Collagenase NB 1 Premium Grade, but Neutral Protease NB can be combined with other collagenases like Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range as well. For applications with GMP requirements, Nordmark offers Neutral Protease NB GMP Grade, which can be used in combination with Collagenase NB 1 GMP Grade. Along with the Collagenase NB product line, Nordmark also offers animal-free products, Neutral Protease AF GMP Grade and Collagenase AF-1 GMP Grade, which are completely free of any animal-based components, thereby excluding the risk of transmission of potential animal-derived pathogens.

Ordering Information

Product NameCat. No. SizePrice
Neutral Protease NBS303011150 DMC* UInquire
Neutral Protease NBS3030112100 DMC* UInquire

Documentation

Safety Information

Signal wordDANGER
Hazard statementsH315 - H317 - H319 - H334 - H335
PrecautionsP280
ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3  • CAS [9001-12-1]

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