Flourescent image of hepatocytes

Isolation of Hepatocytes with Collagenase NB

Protocol Recommendations

In the following, application details for isolation of hepatocytes from rodent, porcine and human liver tissues are presented.

Hepatocytes

SpeciesApplication FieldEnzymesExperimental ConditionsDownloads
HumanResearchCollagenase NB 4G Proved Grade  0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details
HumanClinicCollagenase NB 6 GMP Grade 0.25 - 0.35 PZ U/ml,
10 - 20 min, 37 °C
Details
 MouseResearchCollagenase NB 4G Proved Grade  0.08 - 0.11 PZ U/ml, 
5 - 15 min, 37 °C
Details
PigResearchCollagenase NB 8 Broad Range 0.2 - 0.3 PZ U/mlDetails
Rat (adult)ResearchCollagenase NB 4G Proved Grade  0.12 - 0.15 PZ U/ml, 
10 - 25 min, 37 °C
Details

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Brief Product Information

Collagenase NB 6 GMP Grade
This Collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range 
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Collagenase NB 4G Proved Grade
This product has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation Note

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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